PMID: 1203056Oct 1, 1975Paper

The phosphorylase kinase deficiency (Phk) locus in the mouse: evidence that the mutant allele codes for an enzyme with an abnormal structure

Biochemical Genetics
S R GrossS Pangburn

Abstract

Female (I/St X C57BL/St) F1 mice heterozygous at the sex-linked phosphorylase kinase deficiency locus (Phk) have phosphorylase kinase activities averaging 86% that of mice homozygous for the wild-type allele (C57BL/St), i.e., 72% greater than the sum of one-half the activities of the parental strains. Approximately one-half the phosphorylase kinase activity in the (I X C57BL) F1 muscle extracts had a stability at 42.5 C similar to that of the activity in C57BL extracts (t1/2 = 13.2 min); the other half of the activity in the F1 extracts was more labile (t1/2 = 3.9 min). Two species of phosphorylase kinase activity in F1 muscle extracts were also differentiated with an antiserum prepared in guinea pigs against purified rabbit skeletal muscle phosphorylase kinase. This anti-serum cross-reacted with phosphorylase kinase in C57BL muscle extracts but did not cross-react with skeletal muscle extracts of mice hemi- or homozygous for the mutant allele (I/LnJ). The guinea pig antiserum precipitated 52% as much protein from (I X C57BL)F1 muscle extracts compared to those of C57BL. However, an antiserum prepared against purified rabbit skeletal muscle phosphorylase kinase in the goat cross-reacted with the mutant phosphorylase kinase. The...Continue Reading

References

May 25, 1973·Science·G M Edelman
Jun 1, 1973·Biochemical Genetics·F HuijingD L Coleman
Jun 14, 1968·Annals of the New York Academy of Sciences·D H AlpersG Tomkins
Jan 19, 1971·Biochemistry·K K Lew, J R Roth
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·B Mintz, W W Baker
Oct 1, 1970·FEBS Letters·F Huijing

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Citations

Jan 1, 1980·Journal of Inherited Metabolic Disease·G Bulfield
Jan 1, 1983·Journal of Inherited Metabolic Disease·H C Walvoort
May 1, 1977·The Proceedings of the Nutrition Society·G Bulfield
Nov 1, 1977·Archives of Biochemistry and Biophysics·S R Gross, K Bromwell
Aug 30, 1991·Biochemical and Biophysical Research Communications·P K Bender
Oct 1, 1978·European Journal of Biochemistry·D Daegelen-ProuxJ C Dreyfus
Oct 1, 1990·Annals of Neurology·P R ClemensA G Engel

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