PMID: 19927594Jan 1, 1996Paper

The phosphotransferase activity of cytosolic 5'-nucleotidase; a purine analog phosphorylating enzyme

The International Journal of Biochemistry & Cell Biology
S BanditelliMaria Grazia Tozzi

Abstract

Cytosolic 5'-nucleotidase is involved in the phosphorylation of several purine nucleoside analogs,used as antiviral and chemotherapeutic agents. In order to assess its role in the mechanisms of activation and inactivation of purine prodrugs, it is essential to study the regulation of both hydrolase and phosphotransferase activities of the enzyme. Using a zone capillary electrophoresis apparatus, we were able to separate substrates and products of the reactions catalyzed by cytosolic 5'-nucleotidase. The method overcomes the frequent unavailability of radiolabeled substrates, and allows the influence of possible effectors and/or experimental conditions on both enzyme activities to be evaluated simultaneously. Results showed that the enzyme was able to phosphorylate several nucleosides and nucleoside analogs with the following efficiency: inosine and 2'-deoxyinosine > 2',3'-dideoxyinosine > 6-chloropurineriboside > 6-hydroxylaminepurine riboside> 2,6-diaminopurine riboside > adenosine > cytidine > deoxycoformycin > 2'deoxyadenosine. This is the first report of deoxycoformycin phosphorylation catalyzed by a 5'-nucleotidase purified from eukaryotic cells. The optimum pH for nucleoside monophosphate hydrolysis was 6.5, slightly more...Continue Reading

References

Nov 14, 1979·Biochemical and Biophysical Research Communications·C FriedenR L Miller
Aug 1, 1992·Journal of Biochemical and Biophysical Methods·J M FujitakiR K Robins
Jul 15, 1992·The Biochemical Journal·H Zimmermann
Dec 1, 1991·Archives of Biochemistry and Biophysics·M G TozziP L Ipata
Jan 1, 1991·Advances in Experimental Medicine and Biology·M G TozziP L Ipata
Jul 7, 1987·Biochimica Et Biophysica Acta·R A StinsonH B Collier
Sep 1, 1982·The Biochemical Journal·Y Worku, A C Newby
May 17, 1984·Biochimica Et Biophysica Acta·G TesoriereM Giuliano
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M S HershfieldB F Haynes
Feb 1, 1994·Journal of Biochemical Toxicology·M TurrianiM G Tozzi
Nov 1, 1993·The International Journal of Biochemistry·C BaiocchiP L Ipata
Dec 1, 1993·Journal of Biochemical and Biophysical Methods·S AllegriniM Camici
May 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R Itoh

❮ Previous
Next ❯

Citations

Mar 12, 1998·International Journal of Cancer. Journal International Du Cancer·V BemiM Camici
Sep 12, 2013·Analytical and Bioanalytical Chemistry·Daniela Nicole FiloniMaria Grazia Tozzi
Apr 12, 2008·Purinergic Signalling·Piero Luigi Ipata, Maria Grazia Tozzi
Jun 21, 2005·Pharmacology & Therapeutics·Sally Anne HunsuckerJozef Spychala
Oct 30, 2010·The FEBS Journal·Rossana PesiMaria Grazia Tozzi
Apr 11, 2015·Biochimica Et Biophysica Acta·Federico CividiniMaria Grazia Tozzi
May 1, 2008·Biochimica Et Biophysica Acta·Maria Giovanna CaredduMaria Grazia Tozzi
Feb 7, 2015·Biochemical Pharmacology·F CividiniM G Tozzi
Jun 28, 2020·Nature Communications·Loïc CarriqueNushin Aghajari
Dec 3, 2011·Nucleosides, Nucleotides & Nucleic Acids·Daniela Nicole FiloniMaria Grazia Tozzi
Dec 21, 2004·European Journal of Biochemistry·Simone AllegriniMaria Grazia Tozzi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
R Itoh
Advances in Experimental Medicine and Biology
Maria Grazia TozziPiero Luigi Ipata
© 2022 Meta ULC. All rights reserved