The phylogenetic analysis and putative function of lysine 2,3-aminomutase from methanoarchaea infers the potential biocatalysts for the synthesis of β-lysine

Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi
Chuan-Chuan Hung, Mei-Chin Lai

Abstract

β-amino acids play important biological roles as precursors in the biosynthesis of antibiotics, anticancer agents, neurotransmitters, and other high molecular weight polymers. Microbial cells and enzymes from extreme environments offer new opportunities for biocatalysis and biotransformations as a result of their extreme stability. Lysine 2,3-aminomutase catalyzes the interconversion of L-α-lysine and L-β-lysine. L-β-lysine is a precursor in the bacterial biosynthesis of several antibiotics, and also is a precursor in the biosynthesis of osmolyte N(ε)-acetyl-β-lysine for salt stress and adaptation in methanoarchaea. Lysine 2,3-aminomutase (AblA) genes from the marine Methanosarcina mazei N2M9705, halotolerant Methanocalculus chunghsingensis K1F9705b(T), and halophilic Methanohalophilus portucalensis FDF1(T) were cloned by PCR and southern hybridization. Both nucleotide and amino acid sequences of AblAs were analyzed and phylogenetic comparisons performed. Additionally, the functional motifs and 3D structure of aminomutases were aligned and compared. The deduced amino acid sequences of AblAs from methanoarchaea share high identity with the known clostridial and Bacillus lysine 2,3-aminomutase. The conserved amino acid residues f...Continue Reading

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