The PhyR homolog RSP_1274 of Rhodobacter sphaeroides is involved in defense of membrane stress and has a moderate effect on RpoE (RSP_1092) activity

BMC Microbiology
Qingfeng LiGabriele Klug

Abstract

A major role of the PhyR-NepR-σ(EcfG) cascade in the general stress response was demonstrated for some bacterial species and considered as conserved in Alphaproteobacteria. The σ(EcfG) factor activates its target genes in response to diverse stresses and NepR represents its anti-sigma factor. PhyR comprises a response regulator domain and a sigma factor domain and acts as anti-sigma factor antagonist. The facultative phototrophic alphaproteobacterium Rhodobacter sphaeroides harbours a PhyR homolog in the same genomic context as found in other members of this class. Our study reveals increased expression of the phyR gene in response to superoxide, singlet oxygen, and diamide and also an effect of PhyR on rpoE expression. RpoE has a central role in mounting the response to singlet oxygen in R. sphaeroides. Despite these findings a mutant lacking PhyR was not significantly impeded in resistance to oxidative stress, heat stress or osmotic stress. However a role of PhyR in membrane stress is demonstrated. These results support the view that the effect of the PhyR-NepR-σ(EcfG) cascade on diverse stress responses varies among members of the Alphaproteobacteria. In the facultative phototroph Rhodobacter sphaeroides PhyR plays no major ...Continue Reading

References

May 1, 1991·Journal of Bacteriology·P HübnerT A Bickle
Jul 28, 2004·Journal of Molecular Biology·Jennifer R AnthonyTimothy J Donohue
Apr 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jennifer R AnthonyTimothy J Donohue
Aug 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Benjamin GourionJulia A Vorholt
Jun 1, 2007·Journal of Proteome Research·Jens GlaeserGabriele Klug
Nov 8, 2007·Molecular Microbiology·Cristina E Alvarez-MartinezSuely L Gomes
Nov 21, 2007·Journal of Bacteriology·Benjamin GourionJulia A Vorholt
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anne Francez-CharlotJulia A Vorholt
Feb 26, 2009·Cell·Lauren S Waters, Gisela Storz
Nov 13, 2009·Molecular Microbiology·Bork A BerghoffGabriele Klug
Mar 10, 2010·Current Opinion in Microbiology·Robert B Bourret
Oct 28, 2010·Molecular Microbiology·Anna Staroń, Thorsten Mascher
Jul 5, 2011·Advances in Microbial Physiology·J GlaeserGabriele Klug
Sep 20, 2011·Molecular Cell·Gisela StorzKaren M Wassarman
Sep 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nils N MankGabriele Klug
Oct 3, 2012·PLoS Genetics·Yann S DufourTimothy J Donohue
Mar 22, 2014·RNA Biology·Wolfgang R HessGabriele Klug
Jan 15, 2015·Trends in Microbiology·Anne Francez-CharlotJulia A Vorholt
Oct 8, 2015·Annual Review of Genetics·Aretha FiebigSean Crosson
Oct 16, 2016·The FEBS Journal·Andrea von ZadowGabriele Klug

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Citations

Oct 4, 2019·Environmental Microbiology·Matthew McIntoshGabriele Klug

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Datasets Mentioned

BETA
GSE71844

Methods Mentioned

BETA
PCR
dRNAseq
RNA-seq

Software Mentioned

Quantity One

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