The physical presence of gallstone modulates ex vivo cholesterol crystallization pathways of human bile

Gastroenterology Report
Piero PortincasaDavid Q-H Wang

Abstract

Cholesterol crystallization is an essential step toward gallstone formation. Although model bile studies showed that competition occurs between the gallstone surface and the surrounding aqueous phase for cholesterol molecules available for crystallization, this has not been investigated in human bile. Fresh gallbladder bile was obtained during laparoscopic cholecystectomy from 13 patients with cholesterol (n = 10) or pigment (n = 3) stones. Small cholesterol gallstones were collected from another two patients. Both native and ultrafiltered bile with or without added gallstones was analysed by polarized light microscopy for the presence of arc-like and needle-like anhydrous cholesterol crystals and classic cholesterol monohydrate crystals. Weight of the added stones was evaluated before and after 21 days of bile incubation. In unfiltered bile, the presence of stones was associated with a trend towards less anhydrous cholesterol crystals, but significantly more aggregated cholesterol monohydrate crystals. In ultrafiltered bile, the presence of stones tended to inhibit the formation of arc-like or needle-like crystals and was associated with significantly greater amounts of both plate-like and aggregated cholesterol monohydrate cr...Continue Reading

References

Aug 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·M TakayamaI Tanimizu
May 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D JüngstG Paumgartner
Apr 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A K GroenG N Tytgat
Jun 1, 1973·The Journal of Clinical Investigation·R T HolzbachK Holan
Jun 13, 1983·FEBS Letters·G J Somjen, T Gilat
Mar 1, 1994·Gastroenterology·J R SanabriaS M Strasberg
May 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H S KaufmanK D Lillemoe
Jan 1, 1994·Clinical Science·J H KlinkspoorA K Groen
Jun 1, 1993·The Clinical Investigator·C Wolpers, A F Hofmann
Apr 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P PortincasaG P vanBerge-Henegouwen
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jan 5, 2005·Biochimica Et Biophysica Acta·Niels G VennemanKarel J van Erpecum
May 10, 2005·European Journal of Gastroenterology & Hepatology·Thomas RabensteinHans T Schneider
Jul 18, 2006·Lancet·Piero PortincasaGiuseppe Palasciano
Mar 25, 2011·Korean Journal of Radiology : Official Journal of the Korean Radiological Society·Young Hwan KimTae Beom Shin
Nov 13, 2015·Expert Review of Gastroenterology & Hepatology·P PortincasaD Q-H Wang
Apr 18, 2016·Journal of Hepatology·UNKNOWN European Association for the Study of the Liver (EASL). Electronic address: easloffice@easloffice.eu
Oct 18, 2016·Surgical Laparoscopy, Endoscopy & Percutaneous Techniques·Yang ZhangMingmei Liao
Jun 15, 2017·Surgical Laparoscopy, Endoscopy & Percutaneous Techniques·Xiaokun LinYijiang Han
Dec 29, 2017·Current Opinion in Gastroenterology·Agostino Di CiaulaPiero Portincasa

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