The physiology of the midgut of Lutzomyia longipalpis (Lutz and Neiva 1912): pH in different physiological conditions and mechanisms involved in its control

The Journal of Experimental Biology
Vânia C SantosNelder F Gontijo

Abstract

Nutrient digestion and absorption after blood feeding are important events for Lutzomyia longipalpis, which uses these nutrients to produce eggs. In this context, the pH inside the digestive tract is an important physiological feature as it can markedly influence the digestive process as well as interfere with Leishmania development in infected phlebotomines. It was described previously that unfed females have an acidic midgut (pH 6). In this study, the pH inside the midgut of blood-fed females was measured. The abdominal midgut (AM) pH varied from 8.15+/-0.31 in the first 10 h post-blood meal to 7.7+/-0.17 after 24 h. While the AM was alkaline during blood digestion, the pH in the thoracic midgut (TM) remained acidic (5.5-6.0). In agreement with these findings, the enzyme alpha-glucosidase, which has an optimum pH of 5.8, is mainly encountered in the acidic TM. The capacity of unfed females to maintain the acidic intestinal pH was also evaluated. Our results showed the presence of an efficient mechanism that maintains the pH almost constant at about 6 in the midgut, but not in the crop. This mechanism is promptly interrupted in the AM by blood ingestion. RT-PCR results indicated the presence of carbonic anhydrase in the midgut...Continue Reading

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Citations

Jan 12, 2013·FEMS Microbiology Letters·Anne C S FernandesThaïs Souto-Padrón
Sep 22, 2017·The Journal of Experimental Biology·Denise Barguil NepomucenoNelder Figueiredo Gontijo
Mar 13, 2017·Cell and Tissue Research·Géssica de SousaEdnildo de Alcantara Machado
Apr 15, 2011·The Journal of Experimental Biology·Vânia C SantosNelder F Gontijo
May 6, 2021·Anais Da Academia Brasileira De Ciências·Felipe D Rêgo, Rodrigo Pedro Soares
Nov 13, 2019·Journal of Insect Physiology·Denise Barguil NepomucenoNelder Figueiredo Gontijo
Nov 4, 2021·Insect Science·Denise Barguil NepomucenoNelder Figueiredo Gontijo

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