The phytochrome response of the Lemna gibba NPR1 gene is mediated primarily through changes in abscisic acid levels

Plant Physiology
S C WeatherwaxE M Tobin

Abstract

Two important signaling systems involved in the growth and development of plants, those triggered by the photoreceptor phytochrome and the hormone abscisic acid (ABA), are involved in the regulation of expression of the NPR1 gene of Lemna gibba. We previously demonstrated that phytochrome action mediates changes in ABA levels in L. gibba, correlating with changes in gene expression evoked by stimulation of the phytochrome system. We have now further characterized phytochrome- and ABA-mediated regulation of L. gibba NPR1 gene expression using a transient particle bombardment assay, demonstrating that regulatory elements controlling responses to both stimuli reside within 156 nucleotides upstream of the transcription start. Linker scan (LS) analysis of the region from -156 to -70 was used to identify two specific requisite and nonredundant cis-acting promoter elements between -143 to -135 (LS2) and -113 to -101 (LS5). Mutation of either of these elements resulted in a coordinate loss of regulation by phytochrome and ABA. This suggests that, unlike the L. gibba Lhcb2*1 promoter, in which phytochrome and ABA regulatory elements are separable, the phytochrome response of the L. gibba NPR1 gene can be attributed to alterations in ABA...Continue Reading

References

Feb 11, 1992·Biochemical Society Transactions·W R MarcotteR S Quatrano
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Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R FosterN H Chua

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Citations

Mar 4, 2006·Plant Molecular Biology·Byung-Hoon Kim, Albrecht G von Arnim
Nov 11, 2003·Trends in Plant Science·David P HorvathMichael E Foley
Nov 24, 1999·Trends in Plant Science·A J Cutler, J E Krochko
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Sjef Smeekens
Jul 17, 2001·Physiologia Plantarum·Liming Xiong, Jian-Kang Zhu

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