The Piezo1 cation channel mediates uterine artery shear stress mechanotransduction and vasodilation during rat pregnancy

American Journal of Physiology. Heart and Circulatory Physiology
Liam JohnGeorge Osol

Abstract

During mammalian pregnancy, the uterine circulation must undergo substantial vasodilation and growth to maintain sufficient uteroplacental perfusion. Although we and others have shown that nitric oxide (NO) is a key mediator of these processes, the mechanisms that augment uterine artery NO signaling during gestation have not been identified. We hypothesized that Piezo1, a recently discovered cation channel, may be involved in the process of shear stress mechanotransduction, as other studies have shown that it is both mechanosensitive and linked to NO production. Surprisingly, there are no studies on Piezo1 in the uterine circulation. Our aims in the present study were to determine whether this novel channel is 1) present in uterine arteries, 2) regulated by gestation, 3) functionally relevant (able to elicit rises in intracellular Ca2+ concentration and vasodilation), and 4) linked to NO. Immunohistochemistry confirmed that Piezo1 is present in uterine arteries, primarily but not exclusively in endothelial cells. Western blot analysis showed that its protein expression was elevated during gestation. In pressurized main uterine arteries, pharmacological activation of Piezo1 by Yoda1 produced near maximal vasodilation and was ass...Continue Reading

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Citations

Sep 20, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·David J Beech, Antreas C Kalli
Sep 24, 2019·American Journal of Hypertension·Olufunke O ArisheR Clinton Webb
Aug 28, 2020·Frontiers in Physiology·Etienne RouxThierry Couffinhal
Aug 28, 2019·Annual Review of Pharmacology and Toxicology·Bailong Xiao
Apr 12, 2020·International Journal of Molecular Sciences·Maurizio Mandalà
Apr 2, 2019·Molecular Human Reproduction·L C MorleyN A B Simpson
Aug 9, 2020·Progress in Biophysics and Molecular Biology·Yang GuoOliver Friedrich
Nov 11, 2019·Trends in Pharmacological Sciences·Dominique DouguetEric Honoré
Oct 21, 2021·American Journal of Physiology. Cell Physiology·Ziyi WangJason X-J Yuan

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Methods Mentioned

BETA
protein assay
dissection
Confocal microscopy

Software Mentioned

SigmaPlot verion
ImageJ
WinDaq
IonOptix

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