The pivotal role of micro-environmental cells in a human blood-brain barrier in vitro model of cerebral ischemia: functional and transcriptomic analysis

Fluids and Barriers of the CNS
Anna GerhartlWinfried Neuhaus

Abstract

The blood-brain barrier (BBB) is altered in several diseases of the central nervous system. For example, the breakdown of the BBB during cerebral ischemia in stroke or traumatic brain injury is a hallmark of the diseases' progression. This functional damage is one key event which is attempted to be mimicked in in vitro models. Recent studies showed the pivotal role of micro-environmental cells such as astrocytes for this barrier damage in mouse stroke in vitro models. The aim of this study was to evaluate the role of micro-environmental cells for the functional, paracellular breakdown in a human BBB cerebral ischemia in vitro model accompanied by a transcriptional analysis. Transwell models with human brain endothelial cell line hCMEC/D3 in mono-culture or co-culture with human primary astrocytes and pericytes or rat glioma cell line C6 were subjected to oxygen/glucose deprivation (OGD). Changes of transendothelial electrical resistance (TEER) and FITC-dextran 4000 permeability were recorded as measures for paracellular tightness. In addition, qPCR and high-throughput qPCR Barrier chips were applied to investigate the changes of the mRNA expression of 38 relevant, expressed barrier targets (tight junctions, ABC-transporters) by...Continue Reading

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Citations

Dec 16, 2020·Neural Regeneration Research·Martin A SchickWinfried Neuhaus
Feb 1, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mohammad AbbasJeffrey A Stuart
Apr 18, 2021·Translational Oncology·Robin AugustineAnwarul Hasan

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Methods Mentioned

BETA
Electrophoresis
FCS
PCR
chips
RNA-seq
RNAseq

Software Mentioned

Qlucore Omics Explorer
SigmaPlot

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