The plant-specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity

ELife
Jun QinJie Zhang

Abstract

The vascular pathogen Verticillium dahliae infects the roots of plants to cause Verticillium wilt. The molecular mechanisms underlying V. dahliae virulence and host resistance remain elusive. Here, we demonstrate that a secretory protein, VdSCP41, functions as an intracellular effector that promotes V. dahliae virulence. The Arabidopsis master immune regulators CBP60g and SARD1 and cotton GhCBP60b are targeted by VdSCP41. VdSCP41 binds the C-terminal portion of CBP60g to inhibit its transcription factor activity. Further analyses reveal a transcription activation domain within CBP60g that is required for VdSCP41 targeting. Mutations in both CBP60g and SARD1 compromise Arabidopsis resistance against V. dahliae and partially impair VdSCP41-mediated virulence. Moreover, virus-induced silencing of GhCBP60b compromises cotton resistance to V. dahliae. This work uncovers a virulence strategy in which the V. dahliae secretory protein VdSCP41 directly targets plant transcription factors to inhibit immunity, and reveals CBP60g, SARD1 and GhCBP60b as crucial components governing V. dahliae resistance.

References

Sep 11, 2002·The Plant Journal : for Cell and Molecular Biology·Yule LiuS P Dinesh-Kumar
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sruti DebRoySheng Yang He
Sep 1, 2004·Current Opinion in Plant Biology·Xinnian Dong
May 3, 2005·Annual Review of Plant Biology·Nicolas BouchéHillel Fromm
Jul 10, 2007·The Plant Cell·Bruno DombrechtKemal Kazan
Mar 27, 2009·Plant Physiology·Emilie F FradinBart P H J Thomma
Jun 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christian OttmannClaudia Oecking
Jul 23, 2009·Cellular Microbiology·Haitao CuiJian-Min Zhou
May 22, 2010·BMC Genomics·Dale GodfreyHans Thordal-Christensen
Mar 1, 2006·Molecular Plant Pathology·Emilie F Fradin, Bart P H J Thomma
Sep 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sebastian SchornackEdgar Huitema
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yaxi ZhangYuelin Zhang
Jan 12, 2011·The Plant Journal : for Cell and Molecular Biology·Xiquan GaoLibo Shan
Feb 3, 2011·The Plant Journal : for Cell and Molecular Biology·Marta GodoyRoberto Solano
May 28, 2011·The Plant Journal : for Cell and Molecular Biology·Lin WangJane Glazebrook
May 28, 2011·Plant Physiology·Emilie F FradinBart P H J Thomma
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kinya NomuraSheng Yang He
Oct 7, 2011·Nature·Armin DjameiRegine Kahmann
Mar 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ronnie de JongeBart P H J Thomma
Sep 14, 2012·Molecular Plant-microbe Interactions : MPMI·Parthasarathy Santhanam, Bart P H J Thomma
Oct 12, 2012·Molecular Plant-microbe Interactions : MPMI·Parthasarathy SanthanamBart P H J Thomma
Oct 23, 2012·Cell Host & Microbe·Daolong Dou, Jian-Min Zhou
May 17, 2013·Journal of Integrative Plant Biology·Xiquan GaoLibo Shan

❮ Previous
Next ❯

Citations

Jan 4, 2019·The Plant Journal : for Cell and Molecular Biology·Yuhuan MiaoLongfu Zhu
Feb 13, 2020·International Journal of Molecular Sciences·Ranran SongXingyong Yang
Jul 11, 2018·The Plant Journal : for Cell and Molecular Biology·Xin ZhangYou-Liang Peng
Jul 13, 2019·Frontiers in Plant Science·Xiaowei Han, Regine Kahmann
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yun JinHui-Shan Guo
Dec 1, 2020·Frontiers in Plant Science·Nikhilesh DharSteven J Klosterman
Apr 15, 2021·Molecular Plant-microbe Interactions : MPMI
Apr 14, 2021·Plant Physiology·Lu-Shen LiSha Li
Dec 9, 2020·The Plant Journal : for Cell and Molecular Biology·Karl J SchreiberJennifer D Lewis
Mar 24, 2021·Annual Review of Plant Biology·Yujun PengYuelin Zhang
Apr 24, 2021·Annual Review of Phytopathology·Jie-Yin ChenKrishna V Subbarao
Aug 13, 2021·Journal of Integrative Plant Biology·Muhammad TariqjaveedWenxian Sun
Nov 16, 2021·Plant Communications·Weijie HuangYuelin Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
ChIP-seq
PCR
transfection
transgenic
fluorescence imaging
co-immunoprecipitation
Co-IP
electrophoretic mobility shift assay
electrophoretic mobility shift
confocal microscopy

Software Mentioned

Image J
Mascot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.