The plasma membrane proteome of Saccharomyces cerevisiae and its response to the antifungal calcofluor

Proteomics
Frédéric DelomRémy Gibrat

Abstract

Calcofluor is an antifungal compound known to induce structural perturbations of the cell wall by interfering with the synthesis of chitin microfibril. Proteins from a stripped plasma membrane fraction were solubilized with the neutral and non-denaturing detergent, the n-dodecyl beta-D-maltoside. Proteins were then resolved using a recently described ion-exchange chromatography (IEC)/lithium dodecyl sulfate (LDS)-PAGE procedure. Nearly 90 proteins were identified and clustered, based on their pI, molecular weight, abundance and/or hydrophobicity. This method was then applied to profile the plasma membrane response to calcofluor. The LDS-PAGE patterns obtained from whole plasma membrane proteins were similar for the non-treated and calcofluor-treated samples. However, IEC/LDS-PAGE analysis revealed subtle changes in the expression of several proteins of low abundance, in response to calcofluor. These proteins include Pil1p and Lsp1p, two sphingolipid long-chain base-responsive inhibitors of protein kinases involved in signaling pathways for cell wall integrity and Rho1p, a small GTPase. It was recently hypothesized that Pil1p and Lsp1p could associate with, and regulate, the plasma membrane beta-1-3-glucan synthase, responsible ...Continue Reading

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Citations

Aug 10, 2011·Molecular & Cellular Proteomics : MCP·Aleksandra SzopinskaPierre Morsomme
Dec 29, 2012·Molecular & Cellular Proteomics : MCP·Warren AlbertinDelphine Sicard
Dec 4, 2009·Molecular & Cellular Proteomics : MCP·Elena WiederholdDirk Jan Slotboom
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May 20, 2008·Biochemical and Biophysical Research Communications·Jong KimGregory May
Sep 11, 2009·Proteomics·Thomas PremslerAlbert Sickmann
Sep 10, 2008·Proteomics·Ansgar Poetsch, Dirk Wolters
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Sep 22, 2015·Biotechnology for Biofuels·J SloothaakJ A Tamayo-Ramos
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