The plasma proteins of Biomphalaria glabrata in the presence and absence of Schistosoma mansoni

Developmental and Comparative Immunology
U E ZelckC J Bayne

Abstract

Snail plasma serves as both a sink for metabolites and a source of nutrients for parasites developing within their intermediate hosts. It also contains molecules involved in immunological events like non-self recognition, phagocytosis and encapsulation. In this study we present improved protocols for the separation and partial characterization of plasma proteins of schistosome-susceptible and resistant strains of Biomphalaria glabrata. Within each strain, the plasma of snails 12, 24, 48 and 72 h post-exposure to Schistosoma mansoni and of non-exposed snails was compared. Protein concentrations in hemolymph of all snail strains, non-exposed or parasite-exposed, were about 29 mg/mL and were not found to differ significantly. The dominant plasma molecule (80-85%) is extracellular hemoglobin (Hb) with a native mass of > 1 M Da, and subunits of 190 kDa. It is the only protein bearing heme as shown after separation by native-PAGE and LDS-PAGE. The relatively large amounts of Hb and its large size cause problems if native plasma components are to be separated in PAGE. To obtain satisfactory separation, we used short-term ultracentrifugation to deplete Hb from plasma without qualitative loss of other proteins. Using this methodology, w...Continue Reading

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Citations

Sep 7, 2016·Brazilian Journal of Biology = Revista Brasleira De Biologia·L D SilvaJ C B Bezerra
May 16, 2006·Memórias do Instituto Oswaldo Cruz·João R Coelho, Fernando S M Bezerra
Jan 24, 2019·Acta Parasitologica·Bárbara Brooklyn Timóteo Nascimento SilvaÁlvaro Aguiar Coelho Teixeira
Nov 29, 2016·PLoS Neglected Tropical Diseases·Kulwadee SuwannatraiJavier Sotillo

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