PMID: 9171405Jun 1, 1997Paper

The plasmid R64 thin pilus identified as a type IV pilus

Journal of Bacteriology
S R Kim, T Komano

Abstract

The entire nucleotide sequence of the pil region of the IncI1 plasmid R64 was determined. Analysis of the sequence indicated that 14 genes, designated pilI through pilV, are involved in the formation of the R64 thin pilus. Protein products of eight pil genes were identified by the maxicell procedure. The pilN product was shown to be a lipoprotein by an experiment using globomycin. A computer search revealed that several R64 pil genes have amino acid sequence homology with proteins involved in type IV pilus biogenesis, protein secretion, and transformation competence. The pilS and pilV products were suggested to be prepilins for the R64 thin pilus, and the pilU product appears to be a prepilin peptidase. These results suggest that the R64 thin pilus belongs to the type IV family, specifically group IVB, of pili. The requirement of the pilR and pilU genes for R64 liquid mating was demonstrated by constructing their frameshift mutations. Comparison of three type IVB pilus biogenesis systems, the pil system of R64, the toxin-coregulated pilus (tcp) system of Vibrio cholerae, and the bundle-forming pilus (bfp) system of enteropathogenic Escherichia coli, suggests that they have evolved from a common ancestral gene system.

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Citations

Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Johanna K Hansen, Katrina T Forest
Sep 15, 2012·Molecular Biology and Evolution·Julien GuglielminiEduardo P C Rocha
Dec 24, 2015·Microbial Genomics·Hao Chung TheStephen Baker
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Sep 3, 2011·Foodborne Pathogens and Disease·Jason P FolsterJean M Whichard
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bärbel StecherWolf-Dietrich Hardt
Oct 10, 2013·MicrobiologyOpen·Marleen van WolferenSonja-Verena Albers
Oct 18, 2016·Frontiers in Microbiology·Wenyao ChenChunlei Shi
Feb 20, 2018·Frontiers in Microbiology·Mickaël PoidevinYoshiharu Yamaichi
Jul 14, 2020·Frontiers in Microbiology·María Florencia PerezJulián Rafael Dib
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May 26, 2004·The Journal of Biological Chemistry·Xing-Fu XuYu-Keung Mok
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Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Timothy J Johnson, Lisa K Nolan

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