The Plasmodium translocon of exported proteins component EXP2 is critical for establishing a patent malaria infection in mice

Cellular Microbiology
Ming KalanonTania F de Koning-Ward

Abstract

Export of most malaria proteins into the erythrocyte cytosol requires the Plasmodium translocon of exported proteins (PTEX) and a cleavable Plasmodium export element (PEXEL). In contrast, the contribution of PTEX in the liver stages and export of liver stage proteins is unknown. Here, using the FLP/FRT conditional mutatagenesis system, we generate transgenic Plasmodium berghei parasites deficient in EXP2, the putative pore-forming component of PTEX. Our data reveal that EXP2 is important for parasite growth in the liver and critical for parasite transition to the blood, with parasites impaired in their ability to generate a patent blood-stage infection. Surprisingly, whilst parasites expressing a functional PTEX machinery can efficiently export a PEXEL-bearing GFP reporter into the erythrocyte cytosol during a blood stage infection, this same reporter aggregates in large accumulations within the confines of the parasitophorous vacuole membrane during hepatocyte growth. Notably HSP101, the putative molecular motor of PTEX, could not be detected during the early liver stages of infection, which may explain why direct protein translocation of this soluble PEXEL-bearing reporter or indeed native PEXEL proteins into the hepatocyte c...Continue Reading

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Citations

Jul 5, 2016·Nature Reviews. Microbiology·Tania F de Koning-WardPaul R Gilson
Aug 30, 2016·Cell Reports·Margarida T Grilo RuivoLiliana Mancio-Silva
Sep 28, 2016·International Journal for Parasitology·Samuel Crocodile Wassmer, Georges Emile Raymond Grau
Oct 30, 2016·Trends in Parasitology·Mirko Singer, Friedrich Frischknecht
Jul 3, 2016·International Journal for Parasitology·Paul R GilsonTania F de Koning-Ward
Apr 11, 2018·The FEBS Journal·Scott A ChisholmTania F de Koning-Ward
Sep 3, 2016·FEMS Microbiology Reviews·Emma S Sherling, Christiaan van Ooij
Nov 8, 2020·Nature Communications·João Mello-VieiraMaria M Mota
Apr 2, 2021·PLoS Pathogens·Josh R Beck, Chi-Min Ho
Jul 2, 2021·Frontiers in Cellular and Infection Microbiology·Kayla SylvesterEmily R Derbyshire
Aug 11, 2021·Current Opinion in Microbiology·Matthias Garten, Josh R Beck
Aug 21, 2021·Frontiers in Cellular and Infection Microbiology·Vitomir DjokicNikhat Parveen

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