PMID: 9448329Mar 14, 1998Paper

The plastid ndh genes code for an NADH-specific dehydrogenase: isolation of a complex I analogue from pea thylakoid membranes

Proceedings of the National Academy of Sciences of the United States of America
L A SazanovP J Nixon

Abstract

The plastid genomes of several plants contain ndh genes-homologues of genes encoding subunits of the proton-pumping NADH:ubiquinone oxidoreductase, or complex I, involved in respiration in mitochondria and eubacteria. From sequence similarities with these genes, the ndh gene products have been suggested to form a large protein complex (Ndh complex); however, the structure and function of this complex remains to be established. Herein we report the isolation of the Ndh complex from the chloroplasts of the higher plant Pisum sativum. The purification procedure involved selective solubilization of the thylakoid membrane with dodecyl maltoside, followed by two anion-exchange chromatography steps and one size-exclusion chromatography step. The isolated Ndh complex has an apparent total molecular mass of approximately 550 kDa and according to SDS/PAGE consists of at least 16 subunits including NdhA, NdhI, NdhJ, NdhK, and NdhH, which were identified by N-terminal sequencing and immunoblotting. The Ndh complex showed an NADH- and deamino-NADH-specific dehydrogenase activity, characteristic of complex I, when either ferricyanide or the quinones menadione and duroquinone were used as electron acceptors. This study describes the isolation...Continue Reading

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Citations

May 21, 2011·Journal of Molecular Evolution·Vamsi K Moparthi, Cecilia Hägerhäll
Mar 13, 2010·Photosynthesis Research·Reimund Goss, Torsten Jakob
Nov 16, 2004·Journal of Photochemistry and Photobiology. B, Biology·Maciej GarstkaMałgorzata Rosiak
Apr 19, 2002·Journal of Photochemistry and Photobiology. B, Biology·Nicole A Dijkman, Bernd M A Kroon
Jun 7, 2002·Trends in Plant Science·Thomas HappeAnnette Kaminski
May 11, 1999·Trends in Plant Science·P J Nixon, P Maliga
Nov 14, 2000·Current Opinion in Plant Biology·K K Niyogi
May 21, 2005·Cell Death and Differentiation·J M ZapataB Sabater
Feb 16, 2002·European Journal of Biochemistry·Thomas Happe, Annette Kaminski
Jul 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Pierre Joliot, Giles N Johnson
Oct 16, 2008·The Journal of Biological Chemistry·Lianwei PengToshiharu Shikanai
May 6, 2008·Molecular & Cellular Proteomics : MCP·Wojciech MajeranKlaas J van Wijk
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·L CournacG Peltier
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Nixon
May 15, 2003·Plant Physiology·H Ramiro LascanoBartolomé Sabater
Sep 14, 2002·Annual Review of Plant Biology·Gilles Peltier, Laurent Cournac
Jan 5, 2007·Annual Review of Plant Biology·Toshiharu Shikanai
Apr 13, 2013·Cellular and Molecular Life Sciences : CMLS·Armand G Ngounou WetieCostel C Darie
Feb 4, 2014·Plant Physiology and Biochemistry : PPB·Alexander N Tikhonov
Jan 26, 2016·Biochimica Et Biophysica Acta·John M BerrisfordLeonid A Sazanov
Apr 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Deserah D StrandDavid M Kramer
Nov 6, 2012·Journal of Plant Physiology·Miriam Paredes, María José Quiles
Aug 13, 2011·Current Opinion in Structural Biology·Rouslan G Efremov, Leonid A Sazanov
Feb 23, 2010·Journal of Plant Physiology·Helena IbáñezMaría José Quiles
Sep 19, 2008·Phytochemistry·Chloë van OostendeGilles J C Basset
Mar 23, 2004·Physiologia Plantarum·Maneesha R. Aluru, Steven R. Rodermel

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