The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen development in Arabidopsis.

Plant Physiology
Jesús Muñoz-BertomeuRoc Ros

Abstract

Plant metabolism is highly coordinated with development. However, an understanding of the whole picture of metabolism and its interactions with plant development is scarce. In this work, we show that the deficiency in the plastidial glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPCp) leads to male sterility in Arabidopsis (Arabidopsis thaliana). Pollen from homozygous gapcp double mutant plants (gapcp1gapcp2) displayed shrunken and collapsed forms and were unable to germinate when cultured in vitro. The pollen alterations observed in gapcp1gapcp2 were attributed to a disorganized tapetum layer. Accordingly, the expression of several of the genes involved in tapetum development was down-regulated in gapcp1gapcp2. The fertility of gapcp1gapcp2 was rescued by transforming this mutant with a construct carrying the GAPCp1 cDNA under the control of its native promoter (pGAPCp1::GAPCp1c). However, the GAPCp1 or GAPCp2 cDNA under the control of the 35S promoter (p35S::GAPCp), which is poorly expressed in the tapetum, did not complement the mutant fertility. Mutant GAPCp isoforms deficient in the catalytic activity of the enzyme were unable to complement the sterile phenotype of gapcp1gapcp2, thus confirming that both the expres...Continue Reading

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Citations

May 14, 2014·Journal of Experimental Botany·Julie RipollNadia Bertin
Jul 8, 2014·Trends in Plant Science·Roc RosStephan Krueger
Dec 9, 2014·Plant Signaling & Behavior·Jennifer Selinski, Renate Scheibe
Dec 18, 2012·The Plant Journal : for Cell and Molecular Biology·Doron Shkolnik-InbarDudy Bar-Zvi
Apr 14, 2016·Plant Reproduction·Florian Müller, Ivo Rieu
Jan 21, 2011·Plant Signaling & Behavior·Jesús Muñoz-BertomeuRoc Ros
Mar 31, 2011·Plant Signaling & Behavior·Doron Shkolnik-Inbar, Dudy Bar-Zvi
Dec 5, 2013·Plant Signaling & Behavior·Jesús Muñoz-BertomeuRoc Ros
Dec 17, 2014·Trends in Plant Science·Tzvetina BrumbarovaRumen Ivanov
Sep 8, 2017·International Journal of Molecular Sciences·Do-Yeal RyuMyung-Geol Pang
Apr 28, 2018·PLoS Genetics·Wei ChenYuefeng Guan
Jun 23, 2018·Plant Biology·J Selinski, R Scheibe
Jul 7, 2019·The Plant Journal : for Cell and Molecular Biology·Izabel de Souza ChavesAdriano Nunes-Nesi
Aug 21, 2019·Scientific Reports·Rosane Oliveira NunesMárcia Regina Soares
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haijun JinJia Zhang
Mar 13, 2015·Plant Signaling & Behavior·Jennifer Selinski, Renate Scheibe

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