The platelet-derived growth factor alpha-receptor 1.5 kb transcript: target for molecular detection of testicular germ cell tumours of adolescents and adults

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
J Wolter OosterhuisLeendert H J Looijenga

Abstract

An accurate and relatively simple method to detect testicular germ cell tumours of adolescents and adults (TGCTs) could be useful for early detection and may help to avoid unnecessary orchidectomies. We report a highly sensitive reverse-transcription polymerase chain reaction assay for the 1.5 kb transcript of the platelet-derived growth factor-alpha receptor for molecular diagnosis of TGCTs. As a simulation of the clinical application of the assay the approach was applied on through-cut-biopsies from orchidectomy specimens. In a series of 31 specimens, the 1.5 kb transcript was detected in all samples containing either carcinoma in situ, or an invasive TGCT, with mature teratoma as only exception. No expression was detected in normal parenchyma. On the basis of the through-cut-biopsies the assay shows a sensitivity of 0.87 and a specificity of 1.00. The positive and negative predictive values of the test are 1.00 and 1.00. For carcinoma in situ alone these values are 0.86, 1.00, 1.00, and 0.80, respectively. The figures at least equal the results obtained with the most sensitive morphological/enzyme-histochemical study of duplicate biopsies. We conclude that the 1.5 kb transcript of the platelet-derived growth factor-alpha rec...Continue Reading

References

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Citations

Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·D M de Kretser, I Damjanov
Dec 14, 1999·The American Journal of Pathology·S SiltanenM Heikinheimo
Sep 4, 2009·Prevention Science : the Official Journal of the Society for Prevention Research·Traci M SchwinnJennifer Di Noia
Jul 24, 2010·Endocrine Reviews·Sabrina BascianiLucio Gnessi
Jun 16, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C BokemeyerL H Looijenga

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