The platelet integrin alpha(IIb) beta(3) imaged by atomic force microscopy on model surfaces

Micron : the International Research and Review Journal for Microscopy
Mohammad A Hussain, Christopher A Siedlecki

Abstract

The platelet membrane receptor alpha(IIb) beta(3) binds to adsorbed protein ligands including fibrinogen, von Willebrand factor and fibronectin, and is critically important in mediating platelet adhesion to damaged subendothelium and to synthetic biomaterial surfaces. This receptor is a member of the integrin family, a highly prevalent class of heterodimeric molecules consisting of a single alpha and beta subunit. In an ongoing effort to understand the mechanisms underlying platelet adhesion events, high-resolution atomic force microscopy (AFM) under dynamic conditions was used to obtain images of alpha(IIb) beta(3) molecules as well as aggregates of the protein. Images of integrin molecules were obtained by tapping mode AFM under aqueous buffer conditions following adsorption on a series of ultrasmooth model surfaces. On a model hydrophobic surface, detergents stabilizing the protein in solution competed for surface adsorption sites. When this detergent was removed from the system, the protein was predominantly seen as aggregates with head groups pointing outward. A limited number of individual integrin molecules were observed, and were found to have dimensions consistent with those reported previously by electron microscopy s...Continue Reading

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Citations

Aug 1, 2006·Protein Science : a Publication of the Protein Society·Roy R HantganLeonid Medved
May 14, 2005·Journal of Structural Biology·Kenji IwasakiTakao Yamada
Apr 23, 2013·Biochimica Et Biophysica Acta·Allison M Whited, Paul S-H Park
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Jul 13, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Mohammad A HussainChristopher A Siedlecki

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