The platelet serotonin-release assay

American Journal of Hematology
Theodore E WarkentinJohn G Kelton

Abstract

Few laboratory tests are as clinically useful as The platelet serotonin-release assay (SRA): a positive SRA in the appropriate clinical context is virtually diagnostic of heparin-induced thrombocytopenia (HIT), a life- and limb-threatening prothrombotic disorder caused by anti-platelet factor 4 (PF4)/heparin antibodies that activate platelets, thereby triggering serotonin-release. The SRA's performance characteristics include high sensitivity and specificity, although caveats include indeterminate reaction profiles (observed in ∼4% of test sera) and potential for false-positive reactions. As only a subset of anti-PF4/heparin antibodies detectable by enzyme-immunoassay (EIA) are additionally platelet-activating, the SRA has far greater diagnostic specificity than the EIA. However, requiring a positive EIA, either as an initial screening test or as an SRA adjunct, will reduce risk of a false-positive SRA (since a negative EIA in a patient with a "positive" SRA should prompt critical evaluation of the SRA reaction profile). The SRA also provides useful information on whether a HIT serum produces strong platelet activation even in the absence of heparin: such heparin-"independent" platelet activation is a marker of unusually severe...Continue Reading

References

Nov 1, 1996·The American Journal of Medicine·T E Warkentin, J G Kelton
Feb 12, 1998·Annals of Internal Medicine·T E WarkentinJ G Kelton
Apr 26, 2001·The New England Journal of Medicine·T E Warkentin, J G Kelton
Oct 2, 2001·Annals of Internal Medicine·T E Warkentin, J G Kelton
Oct 24, 2002·Blood·Veronique RegnaultThomas Lecompte
Dec 23, 2004·Journal of Thrombosis and Haemostasis : JTH·J I ZwickerK A Bauer
Feb 24, 2005·American Journal of Hematology·Kathleen SellengTheodore E Warkentin
Nov 29, 2005·The Journal of Laboratory and Clinical Medicine·Theodore E WarkentinJohn G Kelton
Oct 25, 2007·Journal of Thrombosis and Haemostasis : JTH·I SocherU J H Sachs
May 21, 2008·Journal of Thrombosis and Haemostasis : JTH·T E WarkentinJ G Kelton
Jul 1, 2008·The American Journal of Medicine·Theodore E WarkentinJohn G Kelton
Dec 26, 2008·Blood·Andreas GreinacherTheodore E Warkentin
Jan 16, 2009·Blood·Theodore E WarkentinJohn G Kelton
Jan 8, 2010·Thrombosis Research·Marie-Christine Morel-KoppChristopher Ward
Aug 4, 2011·Journal of Thrombosis and Haemostasis : JTH·T E Warkentin
Nov 15, 2011·The American Journal of Medicine·Steven BarolettiSamuel Z Goldhaber
Nov 22, 2011·Seminars in Thrombosis and Hemostasis·Lori-Ann Linkins, Theodore E Warkentin
Dec 14, 2011·Hematology·Theodore E Warkentin
Dec 27, 2011·The American Journal of Medicine·Theodore E Warkentin
Mar 1, 2012·American Journal of Hematology·Theodore E Warkentin
May 26, 2012·Thrombosis and Haemostasis·Theodore E WarkentinDanielle K Griffin
Sep 6, 2012·Journal of Thrombosis and Haemostasis : JTH·T E WarkentinUNKNOWN scientific and standardization committee of the international society on thrombosis and haemostasis
Apr 23, 2013·Therapeutic Advances in Hematology·Tamam Bakchoul, Andreas Greinacher
Jun 1, 2013·Chest·Theodore E WarkentinUNKNOWN Canadian Critical Care Trials Group and the Australian and New Zealand Intensive Care Society Clinical Trials Group

❮ Previous
Next ❯

Citations

Mar 2, 2016·Thrombosis Research·Theodore E Warkentin
Sep 15, 2015·Expert Review of Hematology·Benilde Cosmi
Nov 7, 2015·International Journal of Laboratory Hematology·N K Sono-KoreeK J Smock
Apr 29, 2016·American Journal of Clinical Pathology·Lisa Senzel, Daniel Coldren
May 28, 2016·Journal of the American College of Cardiology·Benjamin S SalterGregory W Fischer
Oct 30, 2016·Thrombosis and Haemostasis·Michael Nagler, Tamam Bakchoul
Sep 15, 2016·Transfusion·Theodore E WarkentinJürgen Bux
Oct 30, 2016·Journal of Molecular Recognition : JMR·Thi-Huong Nguyen
Apr 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valentine MinetFrançois Mullier
May 23, 2017·Nature Communications·Thi-Huong NguyenAndreas Greinacher
Apr 10, 2018·Fukushima Journal of Medical Science·Kiyoshi MigitaSatoru Motokawa
Mar 25, 2016·World Journal of Transplantation·Volker Assfalg, Norbert Hüser
Feb 19, 2019·Journal of Thrombosis and Haemostasis : JTH·Elan MohantyTheodore E Warkentin
Mar 29, 2019·Journal of Thrombosis and Thrombolysis·Tracey ThomasKevin Donahue
Dec 10, 2017·Hematology·Lawrence Rice
Oct 18, 2019·American Journal of Hematology·Theodore E WarkentinDonald M Arnold
Mar 21, 2020·Vascular Medicine·Marie Hogan, Jeffrey S Berger
Mar 14, 2020·Journal of Thrombosis and Haemostasis : JTH·Theodore E WarkentinIshac Nazy
Jul 6, 2016·Current Opinion in Hematology·Theodore E Warkentin, Andreas Greinacher
Nov 6, 2015·Current Opinion in Critical Care·Theodore E Warkentin
Nov 17, 2016·Platelets·Bhanu K ManneMatthew T Rondina
Jul 18, 2017·European Biophysics Journal : EBJ·Thi-Huong Nguyen, Andreas Greinacher
Sep 10, 2020·Current Anesthesiology Reports·Ingrid Moreno-Duarte, Kamrouz Ghadimi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.