The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1.

The EMBO Journal
Julio Sainz de AjaMiguel Manzanares

Abstract

Progenitors of the first hematopoietic cells in the mouse arise in the early embryo from Brachyury-positive multipotent cells in the posterior-proximal region of the epiblast, but the mechanisms that specify primitive blood cells are still largely unknown. Pluripotency factors maintain uncommitted cells of the blastocyst and embryonic stem cells in the pluripotent state. However, little is known about the role played by these factors during later development, despite being expressed in the postimplantation epiblast. Using a dual transgene system for controlled expression at postimplantation stages, we found that Nanog blocks primitive hematopoiesis in the gastrulating embryo, resulting in a loss of red blood cells and downregulation of erythropoietic genes. Accordingly, Nanog-deficient embryonic stem cells are prone to erythropoietic differentiation. Moreover, Nanog expression in adults prevents the maturation of erythroid cells. By analysis of previous data for NANOG binding during stem cell differentiation and CRISPR/Cas9 genome editing, we found that Tal1 is a direct NANOG target. Our results show that Nanog regulates primitive hematopoiesis by directly repressing critical erythroid lineage specifiers.

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Citations

Dec 4, 2019·Biology Open·Antonio BarralJulio Sainz de Aja

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Datasets Mentioned

BETA
GSE71933

Methods Mentioned

BETA
transgenic
FACS
flow cytometry
genotyping
PCR

Software Mentioned

UCSC browser
Enrichr
Ensembl Gene Build
RSEM
GAIIx
limma bioconductor
Cutadapt
UCSC

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