The Pochonia chlamydosporia serine protease gene vcp1 is subject to regulation by carbon, nitrogen and pH: implications for nematode biocontrol.

PloS One
Elaine WardP R Hirsch

Abstract

The alkaline serine protease VCP1 of the fungus Pochonia chlamydosporia belongs to a family of subtilisin-like enzymes that are involved in infection of nematode and insect hosts. It is involved early in the infection process, removing the outer proteinaceous vitelline membrane of nematode eggs. Little is known about the regulation of this gene, even though an understanding of how nutrients and other factors affect its expression is critical for ensuring its efficacy as a biocontrol agent. This paper provides new information on the regulation of vcp1 expression. Sequence analysis of the upstream regulatory region of this gene in 30 isolates revealed that it was highly conserved and contained sequence motifs characteristic of genes that are subject to carbon, nitrogen and pH-regulation. Expression studies, monitoring enzyme activity and mRNA, confirmed that these factors affect VCP1 production. As expected, glucose reduced VCP1 expression and for a few hours so did ammonium chloride. Surprisingly, however, by 24 h VCP1 levels were increased in the presence of ammonium chloride for most isolates. Ambient pH also regulated VCP1 expression, with most isolates producing more VCP1 under alkaline conditions. There were some difference...Continue Reading

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Citations

Feb 18, 2014·Fungal Genetics and Biology : FG & B·Eduardo LarribaLuis Vicente Lopez-Llorca
Nov 23, 2013·Applied Microbiology and Biotechnology·Fabio Ribeiro Braga, Jackson Victor de Araújo
Sep 27, 2016·Environmental Microbiology·Almudena Aranda-MartinezLuis V Lopez-Llorca
Jan 10, 2018·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Ruizhen WangYong-An Zhang
Jan 27, 2021·Environmental Microbiology·Marta Suarez-FernandezLuis Vicente Lopez-Llorca
Apr 4, 2021·International Journal of Molecular Sciences·Marta Suarez-FernandezFederico Lopez-Moya

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Datasets Mentioned

BETA
HE653897
HE653903
AJ427460

Methods Mentioned

BETA
PCR
scraping
PCRs
scanning electron microscopy

Software Mentioned

Primer Express
ClustalX
STADEN
Genstat
Jeol
ABI

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