PMID: 9444997Jan 28, 1998Paper

The pokeweed antiviral protein specifically inhibits Ty1-directed +1 ribosomal frameshifting and retrotransposition in Saccharomyces cerevisiae

Journal of Virology
Nilgun E TumerJonathan D Dinman

Abstract

Programmed ribosomal frameshifting is a molecular mechanism that is used by many RNA viruses to produce Gag-Pol fusion proteins. The efficiency of these frameshift events determines the ratio of viral Gag to Gag-Pol proteins available for viral particle morphogenesis, and changes in ribosomal frameshift efficiencies can severely inhibit virus propagation. Since ribosomal frameshifting occurs during the elongation phase of protein translation, it is reasonable to hypothesize that agents that affect the different steps in this process may also have an impact on programmed ribosomal frameshifting. We examined the molecular mechanisms governing programmed ribosomal frameshifting by using two viruses of the yeast Saccharomyces cerevisiae. Here, we present evidence that pokeweed antiviral protein (PAP), a single-chain ribosomal inhibitory protein that depurinates an adenine residue in the alpha-sarcin loop of 25S rRNA and inhibits translocation, specifically inhibits Ty1-directed +1 ribosomal frameshifting in intact yeast cells and in an in vitro assay system. Using an in vivo assay for Ty1 retrotransposition, we show that PAP specifically inhibits Ty1 retrotransposition, suggesting that Ty1 viral particle morphogenesis is inhibited ...Continue Reading

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Citations

Feb 18, 2003·Molecular and Cellular Biology·Arturas MeskauskasJonathan D Dinman
Jan 9, 2008·The Journal of Biological Chemistry·Bijal A ParikhNilgun E Tumer
Jan 28, 1999·The Journal of Biological Chemistry·K A HudakN E Tumer
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Jul 10, 2012·The Journal of Biological Chemistry·Artem V DomashevskiyDixie J Goss
Sep 14, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Monika SzajwajMarek Tchórzewski
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Sep 10, 2002·Trends in Biochemical Sciences·Jason W HargerJonathan D Dinman
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