The polarity protein Baz forms a platform for the centrosome orientation during asymmetric stem cell division in the Drosophila male germline

ELife
Mayu InabaYukiko M Yamashita

Abstract

Many stem cells divide asymmetrically in order to balance self-renewal with differentiation. The essence of asymmetric cell division (ACD) is the polarization of cells and subsequent division, leading to unequal compartmentalization of cellular/extracellular components that confer distinct cell fates to daughter cells. Because precocious cell division before establishing cell polarity would lead to failure in ACD, these two processes must be tightly coupled; however, the underlying mechanism is poorly understood. In Drosophila male germline stem cells, ACD is prepared by stereotypical centrosome positioning. The centrosome orientation checkpoint (COC) further serves to ensure ACD by preventing mitosis upon centrosome misorientation. In this study, we show that Bazooka (Baz) provides a platform for the correct centrosome orientation and that Baz-centrosome association is the key event that is monitored by the COC. Our work provides a foundation for understanding how the correct cell polarity may be recognized by the cell to ensure productive ACD.

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Citations

Feb 5, 2016·Journal of Developmental Biology·Evan B DeweyChristopher A Johnston
Oct 13, 2015·Current Biology : CB·Tao JiangTony J C Harris
Dec 9, 2015·The Journal of Cell Biology·Athea VichasJennifer A Zallen
Jun 20, 2016·Current Opinion in Cell Biology·Cuie ChenYukiko M Yamashita
Jan 30, 2013·Analytical Biochemistry·Mikhail V KibanovLudmila V Olenina
Oct 26, 2016·Current Biology : CB·Delia RicoloSofia J Araújo
Jan 23, 2016·Molecular Biology of the Cell·Daniel C LevingsHiroshi Nakato
Aug 5, 2017·Journal of Cellular Physiology·Ekaterina Pchelintseva, Mustafa B A Djamgoz
Oct 14, 2017·F1000Research·Tony J C Harris
Mar 14, 2020·PLoS Genetics·Zsolt G VenkeiYukiko M Yamashita
Jan 31, 2018·Genetics·Ryan J GleasonXin Chen
Sep 21, 2018·The Journal of Cell Biology·Zsolt G Venkei, Yukiko M Yamashita
May 26, 2018·Annual Review of Genomics and Human Genetics·Divya JayaramanChristopher A Walsh
Feb 1, 2019·Journal of Cell Science·Kim J A VendelLiedewij Laan
Dec 7, 2020·Current Opinion in Structural Biology·Cayetano Gonzalez
Jun 9, 2021·Oncogene·Farzaneh Atashrazm, Sarah Ellis
Jul 16, 2021·Journal of Molecular Cell Biology·Feifei Qi, Jun Zhou

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Methods Mentioned

BETA
transgenic

Software Mentioned

Excel
ImageJ
GraphPad Prism
Adobe Photoshop

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