The pollen-specific R-SNARE/longin PiVAMP726 mediates fusion of endo- and exocytic compartments in pollen tube tip growth.

Journal of Experimental Botany
Feng Guo, Andrew G McCubbin

Abstract

The growing pollen tube apex is dedicated to balancing exo- and endocytic processes to form a rapidly extending tube. As perturbation of either tends to cause a morphological phenotype, this system provides tractable model for studying these processes. Vesicle-associated membrane protein 7s (VAMP7s) are members of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family that mediate cognate membrane fusion but their role in pollen tube growth has not been investigated. This manuscript identifies PiVAMP726 of Petunia inflata as a pollen-specific VAMP7 that localizes to the inverted cone of transport vesicles at the pollen tube tip. The endocytic marker FM4-64 was found to colocalize with yellow fluorescent protein (YFP)-PiVAMP726, which is consistent with PiVAMP726 containing an amino-acid motif implicated in endosomal localization, At high overexpression levels, YFP- PiVAMP726 inhibited growth and caused the formation of novel membrane compartments within the pollen tube tip. Functional dissection of PiVAMP726 implicated the N-terminal longin domain in negative regulation of the SNARE activity, but not localization of PiVAMP726. Expression of the constitutively active C-terminal SNARE domain alon...Continue Reading

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Citations

Mar 5, 2013·Annual Review of Plant Biology·Caleb M Rounds, Magdalena Bezanilla
Nov 8, 2015·Gene Expression Patterns : GEP·Hongsheng ZhouShaoling Zhang
Oct 27, 2015·Frontiers in Plant Science·Rui MalhóReiaz Ul-Rehman
Sep 30, 2014·Journal of Integrative Plant Biology·Giampiero CaiMauro Cresti
Jan 1, 2013·Plants·Elisabetta Onelli, Alessandra Moscatelli
Nov 9, 2018·Journal of Cell Science·Vasundhara KandacharDusanka Deretic
Jun 24, 2019·The New Phytologist·Liang ZhangJinxing Lin
Dec 1, 2015·Botanical Studies·Pablo Bolaños-VillegasGuang-Yuh Jauh
Nov 15, 2015·Journal of Cell Science·Frédéric DasteDavid Tareste

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Datasets Mentioned

BETA
HM535664
JQ012917

Methods Mentioned

BETA
GTPase
fluorescent
PCR
electrophoresis
2-hybrid
Fluorescence
confocal microscopy
dissection
light microscopy
GTPases

Software Mentioned

BLASTN
BLAST
BLASTP
Adobe Photoshop
ExPASy

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