PMID: 8945520Nov 29, 1996Paper

The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation

Cell
Y TakagakiJ L Manley

Abstract

The switch from membrane-bound to secreted-form IgM that occurs during differentiation of B lymphocytes has long been known to involve regulated processing of the heavy chain pre-mRNA. Here, we show that accumulation of one subunit of an essential polyadenylation factor (CstF-64) is specifically repressed in mouse primary B cells and that overexpression of CstF-64 is sufficient to switch heavy chain expression from membrane-bound (microm) to secreted form (micros). We further show that CstF-64 is limiting for formation of intact CstF, that CstF has a higher affinity for the microm poly(A) site than for the micros site, and that the microm site is stronger in a reconstituted in vitro processing reaction. Our results indicate that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

References

Feb 22, 1991·Cell·N Proudfoot
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y TakagakiJ L Manley
Nov 1, 1989·Genes & Development·Y TakagakiJ L Manley
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L Peterson, R P Perry
Feb 2, 1984·Nature·F R Blattner, P W Tucker
Jul 1, 1995·Trends in Genetics : TIG·K O'Hare
Apr 1, 1995·Current Opinion in Genetics & Development·J L Manley
Jun 16, 1995·Cell·W Keller

❮ Previous
Next ❯

Citations

Jul 1, 2011·Immunologic Research·Patricia SantosChristine Milcarek
May 2, 2013·Trends in Biochemical Sciences·Bin Tian, James L Manley
Feb 28, 2001·Journal of Immunological Methods·P Winding, M W Berchtold
Apr 3, 2001·Molecular Immunology·J L Preud'hommeE Lelièvre
Mar 6, 2003·Molecular Immunology·Christine MilcarekCarol S Lutz
Jun 17, 1999·FEMS Microbiology Reviews·E Wahle, U Rüegsegger
May 9, 2002·Molecular and Cellular Endocrinology·Clinton C MacDonald, José-Luis Redondo
May 8, 1999·Gene·J E Tabaska, M Q Zhang
Dec 28, 1999·Microbes and Infection·D A Lebman, J S Edmiston
May 30, 2012·Cell Research·Martine Simonelig
Nov 4, 2008·Nature·Donny D LicatalosiRobert B Darnell
Jul 24, 2010·Nature Reviews. Immunology·Gary W LitmanSebastian D Fugmann
Sep 29, 2011·Nature Structural & Molecular Biology·Sara A JohnsonDavid L Bentley
Jun 19, 2007·Nature Structural & Molecular Biology·Anita NagHarold G Martinson
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M D KoffaJ B Clements
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·W TangJ S Duke-Cohan
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Valérie HilgersBenjamin Haley
Nov 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chengguo YaoYongsheng Shi
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Béatrice BenoitMartine Simonelig
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·K MartincicC Milcarek
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Audibert, M Simonelig
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A M WallaceC C MacDonald
Dec 2, 2010·Molecular Biology of the Cell·Marc-David RueppDaniel Schümperli
Dec 19, 1998·The EMBO Journal·L Minvielle-SebastiaW Keller
Oct 27, 2012·Journal of Molecular Cell Biology·Yu SunAnlong Xu
Jul 1, 1997·Nucleic Acids Research·G Edwalds-GilbertC Milcarek
Oct 1, 1998·Nucleic Acids Research·K A Sparks, C L Dieckmann
Dec 22, 1999·Nucleic Acids Research·S L ChewI C Eperon
Jan 14, 2005·Nucleic Acids Research·Bin TianCarol S Lutz
Jan 2, 2010·Nucleic Acids Research·Stefania Millevoi, Stéphan Vagner
Jul 14, 2012·Nucleic Acids Research·Michael CowleyRebecca J Oakey

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.