PMID: 4901705Oct 1, 1969Paper

The polyamine content of the tRNA of E. coli

Proceedings of the National Academy of Sciences of the United States of America
S S CohenE Streibel

Abstract

A sensitive method of polyamine estimation has been adapted to the study of the organic cations of small amounts of nucleic acid. A procedure utilizing phenol extraction, alcohol precipitation, and separation on Sephadex G100 has been devised for the isolation of tRNA at low ionic strength. The procedure is applicable to the isolation of tRNA from liter batches of bacterial culture. With these methods we have examined the polyamines of tRNA isolated from polyauxotrophic strains of E. coli incubated under various physiological conditions and have found the following: (1) The tRNA from relaxed bacteria (TAU rel) harvested during exponential growth is heterogeneous with respect to polyamine content. Some portions of the population contain about one mole of spermidine per mole of tRNA. Some putrescine and an unknown amine are also present in low concentration. (2) After exponential TAU rel is incubated with thymine and uracil in the absence of arginine, the tRNA population is far more homogeneous with respect to polyamine content. The various fractions contain two moles of spermidine per mole of tRNA and a small amount of putrescine. (3) After exponential TAU rel is incubated in the absence of both arginine and uracil, the polyamin...Continue Reading

References

Aug 1, 1968·Biochemistry·K H Muench, P A Safille
Dec 1, 1967·European Journal of Biochemistry· FuchseG Walter
Mar 18, 1968·Biochimica Et Biophysica Acta·S Gutcho
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·J R FrescoT Lindahl
Jul 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·C A Buck, M M Nass
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A Raina, S S Cohen
Nov 5, 1965·Journal of the American Chemical Society·T LindahlJ R Fresco
Mar 8, 1966·Biochemical and Biophysical Research Communications·W Szer

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Citations

Dec 1, 1990·Journal of Cellular Biochemistry·R H Davis
Sep 23, 1977·Biochemical and Biophysical Research Communications·T T Sakai, S S Cohen
Apr 6, 1978·Biochimica Et Biophysica Acta·J JänneA Raina
Jan 1, 1996·Metabolism: Clinical and Experimental·Y NoguchiP O Hasselgren
Jul 1, 1975·Nucleic Acids Research·T T SakaiS S Cohen
Sep 19, 2014·Nucleic Acids Research·Helen L Lightfoot, Jonathan Hall
Mar 1, 1995·Experimental Lung Research·J E HeffnerM Jeevanandam
Mar 12, 2010·Plant Physiology and Biochemistry : PPB·Uriel Bachrach
Apr 1, 1980·Toxicology and Applied Pharmacology·D L SpringerF N Dost
Jan 1, 1970·Biochemical and Biophysical Research Communications·R KullnigC Hurwitz
Nov 1, 1979·Medical Hypotheses·D S Dwyer
Apr 7, 1975·Biochemical and Biophysical Research Communications·Y Takeda, T Onishi
Apr 27, 1979·Biochemical and Biophysical Research Communications·R TorgetS S Cohen
Aug 22, 1977·Biochemical and Biophysical Research Communications·P h Janssens de Varebeke, J Augustyniak
Nov 9, 2018·The Journal of Biological Chemistry·André S Bachmann, Dirk Geerts
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·J R ShipeM R Wills
Jul 21, 2020·ACS Infectious Diseases·Vincent MastrodomenicoBryan C Mounce
Nov 7, 1973·Journal of Chromatography·S Ramakrishna, P R Adiga
Aug 1, 1975·Journal of Virology·I Fukuma, S S Cohen
Jun 1, 1992·Microbiological Reviews·R H DavisP Coffino
Dec 1, 1972·Journal of Bacteriology·K E RubensteinS S Cohen
Oct 1, 1977·Infection and Immunity·A A Ibrahim, R Yamamoto

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