The Polyamine Putrescine Contributes to H2 O2 and RbohD/F -Dependent Positive Feedback Loop in Arabidopsis PAMP-Triggered Immunity

Frontiers in Plant Science
Changxin LiuRubén Alcázar

Abstract

Polyamines are involved in defense against pathogenic microorganisms in plants. However, the role of the polyamine putrescine (Put) during plant defense has remained elusive. In this work, we studied the implication of polyamines during pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) in the model species Arabidopsis thaliana. Our data indicate that polyamines, particularly Put, accumulate in response to non-pathogenic Pseudomonas syringae pv. tomato DC3000 hrcC and in response to the purified PAMP flagellin22. Exogenously supplied Put to Arabidopsis seedlings induces defense responses compatible with PTI activation, such as callose deposition and transcriptional up-regulation of several PTI marker genes. Consistent with this, we show that Put primes for resistance against pathogenic bacteria. Through chemical and genetic approaches, we find that PTI-related transcriptional responses induced by Put are hydrogen peroxide and NADPH oxidase (RBOHD and RBOHF) dependent, thus suggesting that apoplastic ROS mediates Put signaling. Overall, our data indicate that Put amplifies PTI responses through ROS production, leading to enhanced disease resistance against bacterial pathogens.

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Citations

Jun 26, 2020·Journal of Experimental Botany·Mohammad Abass AhangerParvaiz Ahmad
Aug 26, 2020·Plant, Cell & Environment·Changxin LiuRubén Alcázar
Oct 11, 2020·Trends in Plant Science·Dylan R ZeissIan A Dubery
Feb 13, 2021·Plant Signaling & Behavior·Changxin Liu, Rubén Alcázar
Jan 7, 2020·Plant Physiology and Biochemistry : PPB·Prabhjot SinglaSatvir K Grewal
Jun 16, 2021·Trends in Plant Science·Léo GerlinStéphane Genin
Aug 15, 2021·Plant Physiology and Biochemistry : PPB·Siyuan HuangYinhua Chen
Oct 2, 2021·Plant Physiology·Theoni MargaritopoulouEmilia Markellou

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

Put
ImageJ
NIS

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