The polyhydroxyalkanoate biosynthesis genes are differentially regulated in planktonic- and biofilm-grown Pseudomonas aeruginosa

Journal of Biotechnology
Andrea CampisanoBernd H A Rehm

Abstract

The planktonic and biofilm growth mode, the presence of polyhydroxyalkanoate (PHA) granules and the nitrogen availability were considered as parameters to study the regulation of genes involved in PHA biosynthesis in Pseudomonas aeruginosa. The transcriptional start point of the phaIF gene cluster, encoding PHA granule-associated proteins with proposed regulatory function, was experimentally verified. Gfp and lacZ transcriptional reporter fusions, respectively, were used to analyse promoter activities. In planktonic growth under nitrogen limitation, the phaC promoter (PphaC) showed increased induction, while in the PHA-negative mutant the activity of PphaC was reduced to 25% of the wild type and was independent of nitrogen availability. Promoter activity in biofilms was assessed using 2, 0.05 or 0 g/l of NH(4)Cl as nitrogen source, respectively. PphaC activity was increased during early biofilm growth, whereas in mature biofilms PphaC activity was preferentially localised to stalks of microcolonies. Nitrogen starvation led to an increased PphaC activity at the biofilm surface. PHA granule formation was confirmed by electron microscopy in planktonic and in biofilm cells. PphaI activity in planktonic cultures was less dependent o...Continue Reading

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Citations

Mar 6, 2010·Journal of Industrial Microbiology & Biotechnology·Slawomir CiesielskiGrzegorz Przybyłek
Nov 4, 2015·Frontiers in Microbiology·Melissa GardinerSuhelen Egan
Jun 11, 2015·Journal of Human Evolution·Marc R MeyerGary J Sawyer
May 24, 2011·Microbiology·Fátima Yousef-CoronadoManuel Espinosa-Urgel
Mar 15, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shuxiong ChenBernd H A Rehm
Mar 21, 2020·Frontiers in Bioengineering and Biotechnology·Jin Xiang WongBernd H A Rehm
Jan 30, 2020·Nature Reviews. Microbiology·M Fata Moradali, Bernd H A Rehm
Jul 18, 2015·Microbiology Spectrum·Olaya Rendueles, Jean-Marc Ghigo
Mar 19, 2014·Journal of Agricultural and Food Chemistry·Luis A GarayJ Bruce German
May 28, 2019·Biomacromolecules·Majela Gonzalez-MiroBernd H A Rehm

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