May 1, 1981

The polyribosomal mRNA--protein complex is a dynamic structure

Proceedings of the National Academy of Sciences of the United States of America
J R Greenberg

Abstract

The metabolism of mRNA--protein complexes present in polyribosomes of mouse L cells was investigated with the aid of ultraviolet light-induced crosslinking of proteins to RNA. It was shown that a set of at least seven proteins which are synthesized and become associated with mRNA under conditions permitting mRNA synthesis also are synthesized and become associated with mRNA when mRNA synthesis is inhibited with a high dose of actinomycin D. This set includes a protein of Mr 78,000 which can be crosslinked to poly(A). These findings imply that mRNA-associated proteins exchange in the cytoplasm with a pool of free proteins. Taken together with results from other laboratories, they suggest a role for mRNA-associated proteins in translation. The labeling kinetics of mRNA-associated proteins were also investigated and found to be consistent with cytoplasmic addition to mRNA and a half-life of more than 2 hr. Additional implications of these findings are discussed.

Mentioned in this Paper

Metabolic Process, Cellular
Polyribosomes
Set of Polysomal Ribosomes
Ribosomal Proteins
Complex (molecular entity)
Protein Complex Location
Staphylococcal Protein A
Cytoplasmic
Metabolic Pathway
L Cells (Cell Line)

About this Paper

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Head And Neck Squamous Cell Carcinoma

Squamous cell carcinomas account for >90% of all tumors in the head and neck region. Head and neck squamous cell carcinoma incidence has increased dramatically recently with little improvement in patient outcomes. Here is the latest research on this aggressive malignancy.

Signaling in Adult Neurogenesis

Neural stem cells play a critical role in the production of neuronal cells in neurogenesis is of great importance. Of interest is the role signalling mechanisms in adult neurogenesis. Discover the latest research on signalling in adult neurogenesis.

Psychiatric Chronotherapy

Psychiatric Chronotherapy considers the circadian rhythm as a major factor for optimizing therapeutic efficacy of psychiatric interventions. Discover the latest research on Psychiatric Chronotherapy here.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.