The positive inotropic effect of angiotensin II: role of endothelin-1 and reactive oxygen species

Hypertension
Horacio E CingolaniErnesto A Aiello

Abstract

Many effects believed to be because of angiotensin II (Ang II) are attributable to the action of endothelin (ET)-1, which is released/produced by Ang II. We investigated whether Ang II elicits its positive inotropic effect (PIE) by the action of endogenous ET-1, in addition to the role played by reactive oxygen species (ROS) in this mechanism. Cat cardiomyocytes were used for: (1) sarcomere shortening measurements; (2) ROS measurements by epifluorescence; (3) immunohistochemical staining for preproET-1, BigET-1, and ET-1; and (4) measurement of preproET-1 mRNA by RT-PCR. Cells were exposed to 1 nmol/L Ang II for 15 minutes. This low concentration of Ang II increases sarcomere shortening by 29.2+/-3.7% (P<0.05). This PIE was abrogated by Na+/H+ exchanger or Na+/Ca2+ exchanger reverse mode inhibition. The production of ROS increased in response to Ang II treatment (DeltaROS respect to control: 68+/-15 fluorescence units; P<0.05). The Ang II-induced PIE and ROS production were blocked by the Ang II type 1 receptor blocker losartan, the nonselective ET-1 receptor blocker TAK044, the selective ETA receptor blocker BQ-123, or the ROS scavenger N-(2-mercapto-propionyl)glycine. Exogenous ET-1 (0.4 nmol/L) induced a similar PIE and incr...Continue Reading

References

Oct 1, 1995·Clinical and Experimental Pharmacology & Physiology·L M DelbridgeP J Harris
Nov 18, 2000·The Journal of Physiology·M G PetroffA Mattiazzi
Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·E A Aiello, H E Cingolani
Feb 5, 2003·Cardiovascular Research·Kouichi TanonakaSatoshi Takeo
Dec 9, 2003·Cardiovascular Research·Néstor G PérezMaría C Camilión de Hurtado
Feb 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ralph V ShohetMasashi Yanagisawa
Jun 4, 2004·Expert Opinion on Investigational Drugs·Ion A Hobai, Brian O'Rourke
Feb 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Irene L EnnisHoracio E Cingolani
Apr 20, 2005·Regulatory Peptides·Horacio E CingolaniMaría C Camilión de Hurtado
Sep 1, 2005·Experimental Eye Research·Vanesa TorbidoniAngela M Suburo
Dec 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Zhiwei YangPedro A Jose

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Citations

Feb 9, 2011·Pflügers Archiv : European journal of physiology·Horacio E CingolaniNéstor G Pérez
Jul 12, 2011·Physiological Reviews·Jeffrey R EricksonMark E Anderson
May 8, 2014·Antioxidants & Redox Signaling·Mark T Ziolo, Steven R Houser
Jan 16, 2007·Canadian Journal of Physiology and Pharmacology·Mackenzie E Malo, Larry Fliegel
Sep 23, 2008·Journal of Hypertension·Pietro Amedeo ModestiAlessandra Modesti
Mar 17, 2010·The Journal of Physiology·María C Villa-AbrilleNéstor G Pérez
Dec 17, 2011·The Journal of Physiology·Claudia I CaldizNéstor G Pérez
Feb 16, 2011·Journal of Molecular and Cellular Cardiology·Anna-Maria KubinIstván Szokodi
Oct 23, 2010·Mechanisms of Ageing and Development·Hirofumi MachiiYasuchika Takeishi
Jul 31, 2010·European Journal of Pharmacology·Takashi KaneshiroYasuchika Takeishi
Oct 27, 2015·Journal of Molecular and Cellular Cardiology·Verónica C De GiustiErnesto A Aiello
Sep 8, 2007·The Journal of Physiology·Claudia I CaldizNéstor G Pérez
Jun 6, 2008·Journal of Cellular Physiology·Solaiman MuftiKlaus-Dieter Schlüter
Feb 12, 2015·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Alejandra M YevesIrene L Ennis
Apr 20, 2014·BioMed Research International·Jacques Noireaud, Ramaroson Andriantsitohaina
Apr 7, 2012·Life Sciences·Nicolas Vignon-ZellwegerNoriaki Emoto
Aug 4, 2009·Journal of Molecular and Cellular Cardiology·Verónica C De GiustiErnesto A Aiello
Sep 4, 2007·American Journal of Hypertension·Vladimir V YurovskyJ Marc Simard
Aug 2, 2017·Antioxidants & Redox Signaling·Amanda J LeBlanc, Natia Q Kelm
Mar 7, 2007·Circulation·Horacio E Cingolani, Irene L Ennis
Jan 8, 2019·Acta Physiologica·Jarrah M DowrickMarie-Louise Ward
Mar 4, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Vincent J de BeerDaphne Merkus
Apr 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Farisa SyedaHaibo Zhang
Sep 20, 2008·Journal of Applied Physiology·Carolina D GarciarenaIrene L Ennis
Jun 10, 2008·The Journal of Pharmacology and Experimental Therapeutics·Qinghua ZengChengwen Sun
Nov 20, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Horacio E CingolaniIrene L Ennis
Oct 5, 2007·The Journal of Biological Chemistry·Nicole DeferCatherine Pavoine
May 29, 2020·Future Cardiology·Nuttha Lumlertgul, Marlies Ostermann
Nov 26, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Osamu YamaguchiYasuchika Takeishi
Oct 12, 2019·Hipertensión y riesgo vascular·A M Yeves, I L Ennis
Mar 12, 2021·Metabolic Brain Disease·Olayemi K IjomoneOmamuyovwi M Ijomone

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