PMID: 3745266Sep 1, 1986Paper

The positive transcription factor of the 5S RNA gene proteolyses during direct exchange between 5S DNA sites

The Journal of Cell Biology
E B Kmiec, A Worcel

Abstract

We have examined the association, dissociation, and exchange of the 5S specific transcription factor (TFIIIA) with somatic- and oocyte-type 5S DNA. The factor associates faster with somatic than with oocyte 5S DNA, and the rate of complex formation is accelerated by vector DNA. Once formed, the TFIIIA-5S DNA complex is stable for greater than 4 h in the absence of free 5S DNA, and its dissociation is identical for somatic and for oocyte 5S DNA. In the presence of free 5S DNA, the factor transfers promptly from the complex to the free 5S DNA site. Unexpectedly, the direct exchange of factor between 5S DNA sites leads to proteolysis at the C-terminal arm of TFIIIA.

References

Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E B KmiecA Worcel
Jun 5, 1986·Journal of Molecular Biology·I Ruberti, A Worcel
Jan 1, 1974·Annual Review of Biochemistry·M J Chamberlin
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J W Little
Jan 25, 1984·Journal of Molecular Biology·M G Fried, D M Crothers
May 1, 1984·Cell·G C GlikinA Worcel

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Citations

Jan 1, 1988·Advances in Enzyme Regulation·M Rechsteiner
Jan 1, 1991·Progress in Biophysics and Molecular Biology·B S Shastry
Nov 13, 2012·Biochimica Et Biophysica Acta·Elodie LayatSylvette Tourmente
Feb 23, 2012·The Plant Journal : for Cell and Molecular Biology·Elodie LayatSylvette Tourmente
Feb 7, 1997·The Journal of Biological Chemistry·S Cho, P C Wensink

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