PMID: 2569Feb 26, 1976

The possibilities and limitations of membrane methods for the histochemical demonstration of cholinesterases

Histochemistry
Z Lukás, Z Fialová

Abstract

The thiocholine method for the histochemical detection of cholinesterases according to Karnovsky-Roots was adapted for unfixed cryostat sections by addition of the agar solution to the incubation mixture and by using the semipermeable membrane interposed between the section and the incubation medium. The procedure prevents the leakage of the enzyme activity of the section and is suitable for tissues where the cholinesterase activity is low.

References

Jul 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P J McMillan, R L Wittum
Dec 31, 1973·Histochemie. Histochemistry. Histochimie·Z Lojda
Mar 1, 1958·The Biochemical Journal·F BERGMANNR SEGAL
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS

Related Concepts

Agar
Metazoa
Cholase
Cornea
Diffusion
Frozen Sections
Cytochemistry
Hydrogen-Ion Concentration
Structure of Intestinal Gland
Membranes, Artificial

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