PMID: 10700651Mar 4, 2000Paper

The possible role of protein-carboxyl methylation in the regulation of flagellar movement of fowl spermatozoa

Animal Reproduction Science
K AshizawaY Tsuzuki


Both intact and demembranated fowl spermatozoa were incubated at 30 degrees C and 40 degrees C with adenosine, 3-deazaadenosine and homocysteine thiolactone. This combination of products is known to block intracellular protein-carboxyl methylation reaction. The motility of intact spermatozoa incubated at 30 degrees C was vigorous but decreased markedly after the addition of 100 microM adenosine+100 microM 3-deazaadenosine+100 microM homocysteine thiolactone. During this incubation period, the intracellular ATP concentrations of spermatozoa were maintained at approximately 40 nmol ATP/10(9) cells, in spite of the inhibition of motility. The motility of demembranated spermatozoa at 30 degrees C was not inhibited by the same concentrations of blocker. At 40 degrees C, the motility of intact spermatozoa without any effectors was almost negligible. The addition of blocker did not appreciably affect the motility of spermatozoa, which remained almost negligible. In contrast, motility became vigorous even at 40 degrees C when intact spermatozoa were suspended in fluid to which had been added 1 mM CaCl(2) or 100 nM calyculin A, a specific inhibitor of protein phosphatase-type 1 and -type 2. Stimulation of motility by Ca(2+) or calyculin...Continue Reading


Jun 15, 1992·Biochemical and Biophysical Research Communications·K AshizawaY Tsuzuki
Aug 1, 1992·Journal of Reproduction and Fertility·K Ashizawa, G J Wishart
Nov 1, 1991·Journal of Reproduction and Fertility·M F Thomson, G J Wishart
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jan 1, 1989·Cell Motility and the Cytoskeleton·J S Tash
Jan 1, 1989·Archives of Andrology·C B Lindemann, K S Kanous
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jul 1, 1987·Journal of Reproduction and Fertility·G J Wishart, K Ashizawa
Nov 1, 1987·Journal of Cellular Biochemistry·C J Brokaw
Nov 1, 1987·Journal of Neurochemistry·P L Vincent, F L Siegel
Apr 15, 1983·Biochemical Pharmacology·B V Sastry, V E Janson
Feb 1, 1981·Fertility and Sterility·D F Katz, J W Overstreet
Sep 1, 1981·The Journal of Experimental Zoology·S Meizel
Aug 1, 1980·The Journal of Cell Biology·P BouchardC W Bardin
Jan 14, 1994·Biochemical and Biophysical Research Communications·M Floer, J Stock
Jan 28, 1998·Molecular Reproduction and Development·K AshizawaY Tsuzuki
Jan 12, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K AshizawaY Tsuzuki

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