The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation

PloS One
Laleh KhodaparastJohan Van Eldere

Abstract

Staphylococcus epidermidis is the most common cause of device-associated infections. It has been shown that active and passive immunization in an animal model against protein SesC significantly reduces S. epidermidis biofilm-associated infections. In order to elucidate its role, knock-out of sesC or isolation of S. epidermidis sesC-negative mutants were attempted, however, without success. As an alternative strategy, sesC was introduced into Staphylococcus aureus 8325-4 and its isogenic icaADBC and srtA mutants, into the clinical methicillin-sensitive S. aureus isolate MSSA4 and the MRSA S. aureus isolate BH1CC, which all lack sesC. Transformation of these strains with sesC i) changed the biofilm phenotype of strains 8325-4 and MSSA4 from PIA-dependent to proteinaceous even though PIA synthesis was not affected, ii) converted the non-biofilm-forming strain 8325-4 ica::tet to a proteinaceous biofilm-forming strain, iii) impaired PIA-dependent biofilm formation by 8325-4 srtA::tet, iv) had no impact on protein-mediated biofilm formation of BH1CC and v) increased in vivo catheter and organ colonization by strain 8325-4. Furthermore, treatment with anti-SesC antibodies significantly reduced in vitro biofilm formation and in vivo co...Continue Reading

References

May 23, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·I RaadK Rolston
Dec 20, 1999·The Journal of Antimicrobial Chemotherapy·E Van WijngaerdenJ Van Eldere
Jul 11, 2001·Journal of Medical Microbiology·J P O'Gara, H Humphreys
Apr 16, 2002·Molecular Microbiology·Friedrich Götz
Oct 7, 2004·Applied and Environmental Microbiology·Emmanuelle CharpentierRichard P Novick
Nov 3, 2004·Biochimica Et Biophysica Acta·Annela M SeddonPaula J Booth
Apr 6, 2005·Research in Microbiology·Shaynoor DramsiHélène Bierne
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Luciano A MarraffiniOlaf Schneewind
Sep 7, 2006·Analytical and Bioanalytical Chemistry·Dietrich MackJohannes K-M Knobloch
Feb 13, 2007·International Journal of Medical Microbiology : IJMM·Susanne HennigWilma Ziebuhr
Apr 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kelly C RiceKenneth W Bayles
Feb 13, 2008·FEMS Microbiology Reviews·Shaynoor DramsiMichel Arthur
Aug 23, 2008·Annals of Medicine·Ilker UçkayFrancis Waldvogel
Sep 2, 2008·Microbiology·Valerie PintensJohan Van Eldere
Jul 18, 2009·Nature Reviews. Microbiology·Michael Otto
Feb 25, 2010·Current Opinion in Infectious Diseases·Gordon Y C Cheung, Michael Otto
Feb 15, 2011·Journal of Biomedicine & Biotechnology·Wouter OosterlinckPaul Herijgers
Jul 18, 2012·Infection and Immunity·Mohammad ShahrooeiJohan Van Eldere
Feb 13, 2015·Frontiers in Cellular and Infection Microbiology·Hannah McCarthyJames P O'Gara
Mar 6, 2015·Frontiers in Cellular and Infection Microbiology·Henning BüttnerHolger Rohde

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Citations

Jul 8, 2016·Revista Da Sociedade Brasileira De Medicina Tropical·Caio Fernando de OliveiraJohanna Ericson Sollid
Nov 18, 2017·Chemical Science·Mary J SabulskiMarcos M Pires
Aug 7, 2017·Journal of Biomedical Materials Research. Part a·Annika JuhlinMargarita Trobos
Dec 13, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Silvestre Ortega-PeñaJuan Carlos Cancino-Diaz
Oct 24, 2019·Molecular Biology Reports·Silvestre Ortega-PeñaJuan C Cancino-Diaz
Jan 23, 2018·Frontiers in Microbiology·Xiuqin QiFangyou Yu
Aug 28, 2020·Frontiers in Microbiology·Timothy J Foster
Feb 10, 2021·Pathogens·Angela FrançaLuís D R Melo

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Methods Mentioned

BETA
reverse transcription-PCR
scanning electron microscopy
dissecting
dissection

Software Mentioned

GraphPad prism

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