The post-replication repair RAD18 and RAD6 genes are involved in the prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine in Saccharomyces cerevisiae

Nucleic Acids Research
Marcelo de PadulaSerge Boiteux

Abstract

7,8-dihydro-8-oxoguanine (8-oxoG) is an abundant and mutagenic lesion produced in DNA exposed to free radicals and reactive oxygen species. In Saccharomyces cerevisiae, the OGG1 gene encodes the 8-oxoG DNA N-glycosylase/AP lyase (Ogg1), which is the functional homologue of the bacterial Fpg. Ogg1-deficient strains are spontaneous mutators that accumulate GC to TA transversions due to unrepaired 8-oxoG in DNA. In yeast, DNA mismatch repair (MMR) and translesion synthesis (TLS) by DNA polymerase eta also play a role in the prevention of the mutagenic effect of 8-oxoG. In the present study, we show the RAD18 and RAD6 genes that are required to initiate post-replication repair (PRR) are also involved in the prevention of mutations by 8-oxoG. Consistently, a synergistic increase in spontaneous CanR and Lys+ mutation rates is observed in the absence of Rad6 or Rad18 proteins in ogg1 mutant strains. Spectra of CaR mutations in ogg1 rad18 and ogg1 rad6 double mutants show a strong bias in the favor of GC to TA transversions, which are 137- and 189-fold higher than in the wild-type, respectively. The results also show that Poleta (RAD30 gene product) plays a critical role on the prevention of mutations at 8-oxoG, whereas Polzeta (REV3 g...Continue Reading

References

Jul 1, 1993·Trends in Genetics : TIG·A P Grollman, M Moriya
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·J P RadicellaS Boiteux
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·A KlunglandD E Barnes
Dec 3, 1999·Science·T Lindahl, R D Wood
Feb 26, 2000·Carcinogenesis·L J Marnett
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·O MinowaT Noda
Apr 25, 2000·Archives of Biochemistry and Biophysics·S Boiteux, J P Radicella
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S TateishiM Yamaizumi
Nov 23, 2000·Nature·T Finkel, N J Holbrook
May 18, 2001·Nature·J H Hoeijmakers
Jul 19, 2001·Mutation Research·S BroomfieldW Xiao
Sep 8, 2001·Molecular Cell·L HaracskaL Prakash
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·K Huebner
Jun 12, 2002·Free Radical Biology & Medicine·Serge BoiteuxNathalie Guibourt
Oct 29, 2003·Molecular Cell·Damien BrégeonPaul W Doetsch

❮ Previous
Next ❯

Citations

Apr 18, 2006·Nucleic Acids Research·Marie GuilletSerge Boiteux
Mar 14, 2009·Nucleic Acids Research·Scott D McCullochThomas A Kunkel
Nov 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Julian E SaleDávid Szüts
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Lauren S WatersGraham C Walker
Jul 28, 2009·Journal of Experimental & Clinical Cancer Research : CR·Tadahiko NakamuraHideo Baba
Aug 13, 2013·PLoS Genetics·Gina P RodriguezGray F Crouse
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·S KozminE Sage
Jul 6, 2013·Drug Development and Industrial Pharmacy·Oliesia Gonzalez QuiñonesMaria Bernadete Riemma Pierre
Aug 11, 2010·Mutation Research·Maria-Elisabetta SerrentinoEvelyne Sage
Jan 29, 2008·DNA Repair·Christophe LavelleLisa Wiesmüller
Aug 24, 2007·DNA Repair·Ayako N SakamotoThomas A Kunkel
Feb 13, 2014·Biochemical and Biophysical Research Communications·Chen XieWu Yue
Apr 2, 2011·Plant Signaling & Behavior·Mayu NakagawaAyako N Sakamoto
May 4, 2005·Yeast
Jun 3, 2020·Journal of Bacteriology·Rupal JainDennis W Grogan
Apr 3, 2013·Genetics·Serge Boiteux, Sue Jinks-Robertson
Aug 23, 2019·Journal of Photochemistry and Photobiology. B, Biology·Raiane R DinizMarcelo de Pádula
Oct 17, 2020·Critical Reviews in Toxicology·Juliana P PaivaMarcelo de Pádula
Feb 2, 2006·Journal of the American Chemical Society·J Carsten PieckThomas Carell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.