PMID: 8582367Sep 1, 1995Paper

The potential of microsatellites for hybridization- and polymerase chain reaction-based DNA fingerprinting of chickpea (Cicer arietinum L.) and related species

Electrophoresis
P C SharmaK Weising

Abstract

The genetic variability in agronomically important chickpea accessions (Cicer arietinum L.) as detected by single-locus restriction fragment length polymorphism (RFLP) probes, random amplified polymorphic DNA (RAPD) and isoenzyme markers, is rather low. Recently, highly polymorphic microsatellites became the markers of choice for linkage mapping and population studies. We are currently following two main strategies to exploit the variability of microsatellites and adjacent sequences for genetic studies in chickpea. (i) In an approach referred to as oligonucleotide fingerprinting, microsatellite-complementary oligonucleotides were employed as multilocus probes for in-gel hybridization. A total of 38 different probes representing di-, tri- and tetranucleotide repeats were used to analyze variability between and within four accessions of C. arietinum. Hybridization signals were obtained with 35 probes. While the abundance and level of polymorphism of different target sequences varied considerably, distinct, intraspecifically informative banding patterns were obtained with the majority of probes and all restriction enzymes tested. No obvious correlation existed between abundance, fingerprint quality, and sequence characteristics of...Continue Reading

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Mar 15, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Niroj Kumar SethySabhyata Bhatia
May 8, 1999·Genome Génome / Conseil National De Recherches Canada·B HüttelG Kahl
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Aug 6, 1999·Electrophoresis·F C Leung
Jul 26, 2002·Journal of Agricultural and Food Chemistry·Jürgen K P Weder

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