The potential of restricted access media columns as applied in coupled-column LC/LC-TSP/MS/MS for the high-speed determination of target compounds in serum. Application to the direct trace analysis of salbutamol and clenbuterol

Analytical Chemistry
E A HogendoornM Montagna

Abstract

This study investigated the potential of restricted access media (RAM) columns used as a first column in coupled-column LC hyphenated to thermospray tandem mass spectrometry (LC/LC-TSP/MS/MS) for the fast, selective, and sensitive determination of target drugs in serum samples. Because of their wide range in polarity, salbutamol and clenbuterol were chosen as model compounds and representatives of the class of beta 2-agonists. Three types of RAM columns were tested: (i) Pinkerton ISRP (internal surface reversed phase, 5 microns), (ii) SPS (semipermeable surface, 5 microns C18), and (iii) RP-18 ADS (alkyl-diol silica, 25 microns). A 3-micron C18 column (50 mm x 4.6 mm i.d.) was chosen as the second column. Tandem mass spectrometric detection was carried out in the selected reaction monitoring (one parent-->one daughter) mode. With regard to retention and, moreover, the peak elution volume of the analytes, the ISRP material was found to perform best: a 50-mm x 4.6-mm i.d. ISRP column in combination with a 100% aqueous buffer (pH of 7.0 +/- 0.2) allowed the injection of large volumes (up to 200 microL) of sample without additional band broadening of the analytes and provided sufficient preseparation between analytes and large-mole...Continue Reading

References

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Citations

Sep 26, 2000·Biomedical Chromatography : BMC·O Y Al-Dirbashi, K Nakashima
Dec 10, 2003·Journal of Chromatography. a·Alida Linda A M StolkerWim Vleeming
Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S SouverainJ-L Veuthey
Oct 25, 2000·Journal of Chromatography. a·E Hogendoorn, P van Zoonen
Jul 13, 2000·Journal of Chromatography. a·F J dos Ramos
Aug 15, 2000·Journal of Chromatography. a·D T Rossi, N Zhang
Sep 24, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·A PolettiniM Montagna
May 29, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·C G GeorgakopoulosM H Spyridaki
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·P Marquet, G Lachâtre
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Mar 29, 2001·Journal of Pharmaceutical and Biomedical Analysis·G MasciV Crescenzi
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gangfeng WangRonald E White
Mar 29, 2002·Rapid Communications in Mass Spectrometry : RCM·Juan V SanchoFélix Hernández
Jun 29, 1999·Analytical Chemistry·D J Anderson

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