The potentiation effect makes the difference: non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro

The Science of the Total Environment
Lingxiangyu LiJose Maria Navas

Abstract

Here we examined whether the addition of a non-toxic concentration (6.25 μg/mL) of zinc oxide nanoparticles (ZnONPs: 19, 35 and 57 nm, respectively) modulates the cytotoxicity of copper nanoparticles (CuNPs, 63 nm in size) in the human hepatoma cell line HepG2. The cytotoxic effect of CuNPs on HepG2 cells was markedly enhanced by the ZnONPs, the largest ZnONPs causing the highest increase in toxicity. However, CuNPs cytotoxicity was not affected by co-incubation with medium containing only zinc ions, indicating the increase in toxicity might be attributed to the particle form of ZnONPs. Transmission electron microscopy (TEM) revealed the presence of CuNPs and ZnONPs inside the cells co-exposed to both types of NP and outflow of cytoplasm through the damaged cell membrane. Inductively coupled plasma mass spectrometry (ICP-MS) determined an increase in the concentration of zinc and a decrease in that of copper in co-exposed cells. On the basis of these results, we propose that accumulation of large numbers of ZnONPs in the cells alters cellular membranes and the cytotoxicity of CuNPs is increased.

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Citations

Mar 11, 2016·Critical Reviews in Toxicology·Jia LiuLongquan Shao
Mar 13, 2016·Environmental Toxicology and Chemistry·David Hernández-MorenoMaría-Luisa Fernández-Cruz
Oct 28, 2016·Environmental Science and Pollution Research International·María BenavidesMário S Diniz
Mar 19, 2020·Critical Reviews in Toxicology·S Keerthana, A Kumar
May 18, 2020·Journal of Toxicology and Environmental Health. Part a·Jéssica Schveitzer KöerichWilliam Gerson Matias
Jun 12, 2019·Materials·Andrei PaduraruBogdan Stefan Vasile
Nov 30, 2020·Journal of Hazardous Materials·Xiaying XinBaiyu Zhang
Jun 13, 2015·Environmental Science & Technology·Tiezheng TongKimberly A Gray
Sep 2, 2021·Water Environment Research : a Research Publication of the Water Environment Federation·María Florencia GutierrezJimena Cazenave
May 1, 2020·Ecotoxicology and Environmental Safety·Xiaoyu LiXiaolan Zeng
Jun 20, 2020·Ecotoxicology and Environmental Safety·Yuting QianSijie Lin

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