The POTRA domains of Toc75 exhibit chaperone-like function to facilitate import into chloroplasts

Proceedings of the National Academy of Sciences of the United States of America
Patrick K O'NeilDanny J Schnell

Abstract

Protein trafficking across membranes is an essential function in cells; however, the exact mechanism for how this occurs is not well understood. In the endosymbionts, mitochondria and chloroplasts, the vast majority of proteins are synthesized in the cytoplasm as preproteins and then imported into the organelles via specialized machineries. In chloroplasts, protein import is accomplished by the TOC (translocon on the outer chloroplast membrane) and TIC (translocon on the inner chloroplast membrane) machineries in the outer and inner envelope membranes, respectively. TOC mediates initial recognition of preproteins at the outer membrane and includes a core membrane channel, Toc75, and two receptor proteins, Toc33/34 and Toc159, each containing GTPase domains that control preprotein binding and translocation. Toc75 is predicted to have a β-barrel fold consisting of an N-terminal intermembrane space (IMS) domain and a C-terminal 16-stranded β-barrel domain. Here we report the crystal structure of the N-terminal IMS domain of Toc75 from Arabidopsis thaliana, revealing three tandem polypeptide transport-associated (POTRA) domains, with POTRA2 containing an additional elongated helix not observed previously in other POTRA domains. Fun...Continue Reading

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Citations

Mar 1, 2018·Annual Review of Plant Biology·Eva C M Nowack, Andreas P M Weber
Apr 24, 2018·Plant & Cell Physiology·Masato Nakai
Jun 27, 2018·Photosynthesis Research·Bettina Bölter
Jun 13, 2019·The Protein Journal·Yuanyuan ChenRoss E Dalbey
Nov 9, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaodan MaGuoyu Meng
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Jan 11, 2020·Biochemical Society Transactions·Simon M ThomsonR Paul Jarvis
Mar 1, 2018·Essays in Biochemistry·Serena SchwenkertJürgen Soll
Aug 28, 2020·Journal of Experimental Botany·Tim JiangRongmin Zhao
Nov 3, 2020·Molecular Plant·Jing-Liang SunJian-Xiang Liu
Dec 15, 2020·Molecular Microbiology·Karl LundquistNicholas Noinaj
Feb 28, 2021·Journal of Molecular Biology·Kathryn A DiederichsIstvan Botos
Apr 19, 2021·Trends in Cell Biology·Madeline M DavisBarry D Bruce
May 27, 2021·The Journal of Cell Biology·Yakubu Princely AbuduTerje Johansen

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