The potyviral virus genome-linked protein VPg forms a ternary complex with the eukaryotic initiation factors eIF4E and eIF4G and reduces eIF4E affinity for a mRNA cap analogue

The FEBS Journal
Thierry MichonO Le Gall

Abstract

The virus protein linked to the genome (VPg) of plant potyviruses is a 25-kDa protein covalently attached to the genomic RNA 5' end. It was previously reported that VPg binds specifically to eIF4E, the mRNAcap-binding protein of the eukaryotic translation initiation complex. We performed a spectroscopic study of the interactions between lettuce eIF4E and VPg from lettuce mosaic virus (LMV). The cap analogue m7GDP and VPg bind to eIF4E at two distinct sites with similar affinity (K(d) = 0.3 microm). A deeper examination of the interaction pathway showed that the binding of one ligand induces a decrease in the affinity for the other by a factor of 15. GST pull-down experiments from plant extracts revealed that VPg can specifically trap eIF4G, the central component of the complex required for the initiation of protein translation. Our data suggest that eIF4G recruitment by VPg is indirectly mediated through VPg-eIF4E association. The strength of interaction between eIF4E and pep4G, the eIF4E-binding domain on eIF4G, was increased significantly by VPg. Taken together these quantitative data show that VPg is an efficient modulator of eIF4E biochemical functions.

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Citations

Oct 13, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kai-Shu LingMichael J Havey
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Laurent VolponKatherine L B Borden
Jul 11, 2008·The Journal of Biological Chemistry·Sergey V SlepenkovRobert E Rhoads
Mar 12, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Martin Carroll, Katherine L B Borden
Jun 24, 2011·Journal of Virology·Katri EskelinKristiina Mäkinen
Feb 18, 2009·Virology Journal·Eugénie HébrardDenis Fargette
Dec 4, 2012·Viruses·Jiri SochorRene Kizek
Mar 24, 2012·Current Opinion in Virology·Jun Jiang, Jean-François Laliberté
Jan 10, 2008·The Plant Journal : for Cell and Molecular Biology·Carine CharronCarole Caranta
Mar 1, 2007·Molecular Plant Pathology·Andrew J MauleMargaret I Boulton
Aug 19, 2008·Molecular Plant Pathology·Sylvie German-RetanaOlivier Le Gall
May 22, 2007·Plant Biotechnology Journal·Byoung-Cheorl KangMolly M Jahn
Nov 12, 2010·The Journal of General Virology·Allan OlspertErkki Truve
Dec 17, 2014·Immunological Reviews·Michael J Osborne, Katherine L B Borden
Apr 17, 2013·Cell Cycle·Laurent VolponKatherine L B Borden
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Apr 12, 2014·Frontiers in Plant Science·Kristiina Mäkinen, Anders Hafrén
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Feb 16, 2017·Genetics and Molecular Biology·João Paulo B MachadoElizabeth P B Fontes
Oct 14, 2017·Molecular Biology and Evolution·Justine CharonThierry Michon
Feb 13, 2020·Plants·Kristin WidyasariKook-Hyung Kim

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