PMID: 9185568Jul 1, 1997Paper

The POU-domain factor Brn-3.0 recognizes characteristic sites in the herpes simplex virus genome

Nucleic Acids Research
E E TurnerL T Feldman

Abstract

The restriction of herpes virus latency to mammalian sensory ganglia has led to a search for tissue-specific regulatory molecules in these neurons which alter viral gene expression. We have recently shown that the POU-domain transcriptional regulator Brn-3.0 is abundantly expressed in the adult trigeminal ganglion. To begin to examine the hypothesis that Brn-3.0 might participate in the regulation of the HSV life-cycle, we used Brn-3.0 POU-domain protein as an affinity matrix, and biochemically screened the entire HSV genome for sites of Brn-3.0 binding. This screen identified several sites of the form TA/TA A T N A N TA/T, which significantly do not include the previously identified HSV octamer sequences. All of the selected sites occur in the <25% of the HSV genome which has not been assigned to open reading frames, suggesting that these sites may be transcriptional regulatory elements recognized by Brn-3.0 or another homeobox factor with similar DNA binding properties. However, these sites do not interact with Brn-3.0 with sufficiently high affinity to directly mediate transcriptional activation by Brn-3.0 alone in transfection assays. The experiments described also provide an effective general method for exhaustive screenin...Continue Reading

References

Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J L McKnightB Roizman
Jul 1, 1988·The Journal of General Virology·D J McGeochP Taylor
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T Gerster, R G Roeder
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M R GerreroM G Rosenfeld
Nov 1, 1995·Mechanisms of Development·N G Fedtsova, E E Turner

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Citations

Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Jin ZhouZhihua Liu
Jan 15, 2003·Clinical Microbiology Reviews·Clinton Jones
Feb 20, 2010·Interdisciplinary Perspectives on Infectious Diseases·Guey-Chuen Perng, Clinton Jones
Feb 24, 2001·Journal of Molecular Biology·K Phillips, B Luisi
Feb 7, 2004·The Journal of Biological Chemistry·Sheng-Hao ChaoJeremy S Caldwell
Jun 18, 1999·Reviews in Medical Virology·D S Latchman

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