Abstract
Microarrays permit biologists to simultaneously measure the mRNA abundance of thousands of genes. An important issue facing investigators planning microarray experiments is how to estimate the sample size required for good statistical power. What is the projected sample size or number of replicate chips needed to address the multiple hypotheses with acceptable accuracy? Statistical methods exist for calculating power based upon a single hypothesis, using estimates of the variability in data from pilot studies. There is, however, a need for methods to estimate power and/or required sample sizes in situations where multiple hypotheses are being tested, such as in microarray experiments. In addition, investigators frequently do not have pilot data to estimate the sample sizes required for microarray studies. To address this challenge, we have developed a Microrarray PowerAtlas. The atlas enables estimation of statistical power by allowing investigators to appropriately plan studies by building upon previous studies that have similar experimental characteristics. Currently, there are sample sizes and power estimates based on 632 experiments from Gene Expression Omnibus (GEO). The PowerAtlas also permits investigators to upload thei...Continue Reading
References
Feb 17, 2000·Nature·A A AlizadehL M Staudt
Mar 22, 2001·The Journal of Biological Chemistry·A D LongP Baldi
May 18, 2001·Genetical Research·M K Kerr, G A Churchill
May 31, 2001·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·M K KerrG A Churchill
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Oct 22, 2002·Science·Catherine A BallUNKNOWN Microarray Gene Expression Data (MGED) Society
Nov 19, 2002·Statistics in Medicine·Mei-Ling Ting Lee, G A Whitmore
Nov 20, 2003·Nutrition·Grier P PageDavid B Allison
Feb 12, 2004·Arthritis and Rheumatism·Huang-Ge ZhangJohn D Mountz
Sep 2, 2004·Nature Genetics·Tapan MehtaDavid B Allison
Dec 8, 2004·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Sue-Jane Wang, James J Chen
Citations
Jan 6, 2010·EMBO Molecular Medicine·Shyra J MillerNancy Ratner
Sep 12, 2006·Nature Biotechnology·Richard ShippyDamir Herman
Apr 19, 2011·Briefings in Bioinformatics·Zhide Fang, Xiangqin Cui
Nov 19, 2008·Bioinformatics·Adam L AsareVicki Seyfert-Margolis
Jun 17, 2009·Bioinformatics·Kathleen F Kerr
Dec 17, 2008·Journal of Applied Physiology·David L AllenJeremy L Barth
Apr 28, 2009·BMC Genomics·Nelida Rodriguez-OsorioErdogan Memili
Nov 11, 2010·BMC Cancer·Beth A TamburiniJaime F Modiano
Jun 5, 2010·PLoS Computational Biology·Mark Reimers
Jun 21, 2008·PLoS Genetics·Gary L GadburyDavid B Allison
May 23, 2009·PloS One·Beth A TamburiniJaime F Modiano
Aug 11, 2011·PloS One·Nicholas F MarkoRobert J Weil
Aug 6, 2011·PloS One·Cheng-Wei ChangIan C Hsu
Jun 6, 2008·International Journal of Plant Genomics·Grier P Page, Issa Coulibaly
Mar 18, 2010·The Journal of Nutritional Biochemistry·Wenjiang J FuGuoyao Wu
Aug 31, 2006·Journal of Leukocyte Biology·Malavika S GiriLuis J Montaner
May 11, 2016·Hypertension·Katherine LeaveyBrian J Cox
Dec 29, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Anna ContiAntonio Faiella
May 15, 2008·BJU International·Bin K KroonLaura J van't Veer
Oct 22, 2008·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Tania DasBanerjeeStephen V Faraone
Sep 20, 2017·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Michael S BreenStephen J Glatt
May 21, 2014·G3 : Genes - Genomes - Genetics·Amy R EllisonKelly R Zamudio
Apr 26, 2019·Scientific Reports·K L Bowlt BlacklockM Starkey
Oct 24, 2009·Biological Psychiatry·Masashi IkedaNakao Iwata
Feb 5, 2014·Cold Spring Harbor Protocols·Manuel Ares