PMID: 7537397Apr 27, 1995Paper

The predictive value of epitope analysis in highly sensitized patients awaiting renal transplantation

Transplantation
G J Laundy, B A Bradley

Abstract

The accumulation of highly sensitized patients (HSP) on renal transplant waiting lists is a problem faced by all transplant registries. We have studied the HLA class I serological reactivity of 20 random HSP and have related antibody specificity to primary amino acid sequence. In six patients we identified significant correlations (chi 2 test, r > or = 0.93) between panel reactivity and specific amino acid substitutions characteristic of HLA-A, -B, and -C public epitopes. Antibody reactivity was associated with up to three public epitopes in each patient. The 12 separate antibody specificities identified were associated with 10 residues. Seven correlated with HLA-A locus substitutions (Glu-62/Gly-65, Lys-66, Arg-114, His-114/Tyr-116/Lys-127, Thr-142/His-145 [x2], and Thr-149), two with HLA-B locus substitutions (Thr-24, Ser-24) and three with interlocus antibodies associated with either HLA-A and B (Leu-82/Arg-83 [x2]) or with HLA-B and -C substitutions (Leu-163). This information allowed us to predict HLA class I allelic products of known primary sequence that would react negatively with each HSP serum. Windows of acceptable mismatches (WAMMs) can thus be delineated with a view to crossmatch negative transplantation without th...Continue Reading

Citations

Jul 5, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Gertie J OostinghCraig J Taylor
Sep 24, 1998·Transplantation Proceedings·R J Duquesnoy
Dec 19, 2006·Transplantation Proceedings·C SpiridonA Nikaein
Dec 2, 2008·Clinical Transplantation·Camelia SpiridonMichael Mack
Dec 18, 2004·Human Immunology·Steve TakemotoRene J Duquesnoy

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