PMID: 9636362Jun 24, 1998Paper

The predominant elF4G-specific cleavage activity in poliovirus-infected HeLa cells is distinct from 2A protease

Virology
M BoveeR E Lloyd

Abstract

Human enteroviruses and rhinoviruses rapidly and selectively abolish translation from cellular mRNA upon infection of susceptible cells. Expression of the poliovirus 2A protease (PV 2Apro) is sufficient to cause host translation shutoff through cleavage of elF4G (formerly p220, elF4 gamma) either directly or indirectly through activation of a cellular factor. Evidence exists for both direct and indirect cleavage mechanisms; however, factors presumed to participate in an indirect mechanism have not yet been purified or defined. Here we show that the dominant elF4G cleavage activity in lysates from infected HeLa cells was separable from PV 2Apro by size exclusion chromatography. 2Apro separated into two peak fractions which contained activity which cleaved a peptide substrate derived from the poliovirus polyprotein. These peak 2Apro fractions did not cleave elF4G or an elF4G-derived peptide, as expected, due to the poor efficiency of direct cleavage reactions. Conversely, fractions which contained peak elF4G cleavage activity and only trace amounts of 2Apro efficiently cleaved a peptide substrate derived from the previously mapped elF4G cleavage site and also cleaved a peptide derived from the poliovirus 1D2A region. The dominant...Continue Reading

References

Nov 1, 1985·Molecular and Cellular Biology·H D BernsteinD Baltimore
Sep 1, 1996·Microbiological Reviews·G J Belsham, N Sonenberg

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Citations

Nov 30, 2007·Journal of Virology·Nogi ParkKurt E Gustin
Feb 12, 2002·Journal of Virology·N Muge Kuyumcu-MartinezRichard E Lloyd
Jul 16, 2004·Journal of Virology·Muge Kuyumcu-MartinezRichard E Lloyd
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D S Lyles
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
May 19, 2012·PloS One·Victoria A MeliopoulosRalph A Tripp
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A GradiN Sonenberg
Dec 31, 2011·The Journal of Infectious Diseases·Soo-Hyeon YunEun-Seok Jeon
Apr 20, 2002·Current Opinion in Infectious Diseases·G Frisk
Dec 30, 2006·Apoptosis : an International Journal on Programmed Cell Death·David H W ChauDecheng Yang
Jan 23, 2016·Life·Suki Albers, Andreas Czech
Sep 20, 2015·Protein Science : a Publication of the Protein Society·Martina AumayrTim Skern
Dec 24, 2015·Viruses·Prabuddha S PathinayakePeter A B Wark
Jan 30, 2004·Journal of Medical Virology·Suvi RasilainenTimo Otonkoski
Nov 24, 2005·Virus Research·Richard E Lloyd
Nov 17, 2007·Cell Host & Microbe·James P WhiteRichard E Lloyd
Sep 14, 2002·The Journal of Biological Chemistry·Nicole FoegerTim Skern
Sep 13, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiangle ZhangHaixue Zheng
Oct 3, 1999·Virus Research·J SeipeltT Skern

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