The predominant molecular state of bound enzyme determines the strength and type of product inhibition in the hydrolysis of recalcitrant polysaccharides by processive enzymes.

The Journal of Biological Chemistry
Silja KuuskPriit Väljamäe

Abstract

Processive enzymes are major components of the efficient enzyme systems that are responsible for the degradation of the recalcitrant polysaccharides cellulose and chitin. Despite intensive research, there is no consensus on which step is rate-limiting for these enzymes. Here, we performed a comparative study of two well characterized enzymes, the cellobiohydrolase Cel7A from Hypocrea jecorina and the chitinase ChiA from Serratia marcescens. Both enzymes were inhibited by their disaccharide product, namely chitobiose for ChiA and cellobiose for Cel7A. The products behaved as noncompetitive inhibitors according to studies using the (14)C-labeled crystalline polymeric substrates (14)C chitin nanowhiskers and (14)C-labeled bacterial microcrystalline cellulose for ChiA and Cel7A, respectively. The resulting observed Ki (obs) values were 0.45 ± 0.08 mm for ChiA and 0.17 ± 0.02 mm for Cel7A. However, in contrast to ChiA, the Ki (obs) of Cel7A was an order of magnitude higher than the true Ki value governed by the thermodynamic stability of the enzyme-inhibitor complex. Theoretical analysis of product inhibition suggested that the inhibition strength and pattern can be accounted for by assuming different rate-limiting steps for ChiA an...Continue Reading

References

Dec 15, 1994·Structure·A PerrakisC E Vorgias
Jul 1, 1996·Microbiology·M B BrurbergV G Eijsink
Oct 23, 1997·Nature Structural Biology·J SakonP A Karplus
Sep 11, 2003·Biotechnology and Bioengineering·Priit VäljamäeGunnar Johansson
Dec 4, 2003·Journal of the American Chemical Society·W Bruce Turnbull, Antonio H Daranas
May 31, 2008·Biotechnology and Bioengineering·Sanni P VoutilainenAnu Koivula
Jul 1, 1990·Biotechnology and Bioengineering·M HoltzappleC Hendrickson
Jul 7, 2009·Biotechnology Advances·Prabuddha BansalAndreas S Bommarius
Mar 9, 2010·Angewandte Chemie·James M MacdonaldStephen G Withers
May 28, 2010·Biotechnology and Bioengineering·Jürgen Jalak, Priit Väljamäe
Nov 6, 2010·The Journal of Biological Chemistry·Mihhail Kurasin, Priit Väljamäe
Mar 5, 2011·The FEBS Journal·Eigil PraestgaardPeter Westh
Jun 15, 2011·Journal of the American Chemical Society·Shishir P S ChundawatBruce E Dale
Sep 3, 2011·Science·Kiyohiko IgarashiMasahiro Samejima
Nov 1, 2011·Biotechnology for Biofuels·Martin J BaumannPeter Westh
Nov 24, 2011·The Journal of Biological Chemistry·Leigh MurphyPeter Westh
Apr 12, 2012·The Journal of Biological Chemistry·Nicolaj Cruys-BaggerPeter Westh
May 23, 2012·Methods in Enzymology·Svein J HornVincent G H Eijsink
Jun 1, 2012·The Journal of Biological Chemistry·Lintao BuGregg T Beckham
Jun 27, 2012·The Journal of Biological Chemistry·Jürgen JalakPriit Väljamäe
Feb 16, 2013·Enzyme and Microbial Technology·Leigh MurphyPeter Westh
Jun 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Dahai GaoBruce E Dale
Jul 26, 2013·Biotechnology for Biofuels·Hele Teugjas, Priit Väljamäe
Sep 24, 2013·Biotechnology for Biofuels·Riin KontPriit Väljamäe

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Citations

Jan 30, 2016·The Journal of Physical Chemistry. B·Suvamay JanaChristina Marie Payne
Aug 25, 2015·Biotechnology and Bioengineering·Meera E AtreyaDouglas S Clark
Jul 15, 2015·The Journal of Physical Chemistry. B·Anne Grethe HamreMorten Sørlie
Dec 5, 2015·Biotechnology and Bioengineering·Johan P OlsenPeter Westh
Jun 2, 2016·The Journal of Physical Chemistry. B·Nicolaj Cruys-BaggerPeter Westh
Jan 28, 2017·PloS One·Silja KuuskPriit Väljamäe
Jul 5, 2017·Biochemical Society Transactions·Adélaïde Raguin, Oliver Ebenhöh
Jan 6, 2017·The Journal of Biological Chemistry·Tian LiuQing Yang
Aug 26, 2020·Journal of Industrial Microbiology & Biotechnology·Heidi ØstbyAnikó Várnai
Nov 2, 2017·Physical Chemistry Chemical Physics : PCCP·Akihiko NakamuraRyota Iino
Nov 16, 2017·The Journal of Biological Chemistry·Silja KuuskPriit Väljamäe
Aug 3, 2021·The Journal of Biological Chemistry·Zachary K HavilandWilliam O Hancock
Dec 28, 2016·Biochemistry·Jeppe KariPeter Westh

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