The preferred route for the degradation of silencing target RNAs in transgenic plants depends on pre-established silencing conditions

Nucleic Acids Research
Matthew SandersJohn Jacobs

Abstract

RNA silencing can be initiated upon dsRNA accumulation and results in homology-dependent degradation of target RNAs mediated by 21-23 nt small interfering RNAs (siRNAs). These small regulatory RNAs can direct RNA degradation via different routes such as the RdRP/Dicer- and the RNA-induced silencing complex (RISC)-catalysed pathways. The relative contribution of both pathways to degradation of target RNAs is not understood. To gain further insight in the process of target selection and degradation, we analysed production of siRNAs characteristic for Dicer-mediated RNA degradation during silencing of mRNAs and chimeric viral RNAs in protoplasts from plants of a transgenic tobacco silencing model line. We show that small RNA accumulation is limited to silencing target regions during steady-state mRNA silencing. For chimeric viral RNAs, siRNA production appears dependent on pre-established cellular silencing conditions. The observed siRNA accumulation profiles imply that silencing of viral target RNAs in pre-silenced protoplasts occurs mainly via a RISC-mediated pathway, guided by (pre-existing) siRNAs derived from cellular mRNAs. In cells that are not silenced at the time of infection, viral RNA degradation seems to involve Dicer ...Continue Reading

References

Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Aug 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ulrich KlahreFrederick Meins
Jan 7, 2003·Genes & Development·Guiliang TangPhillip D Zamore
Jan 17, 2003·Plant Physiology·Helena Van HoudtAnna Depicker
Jan 30, 2003·RNA·Paula SteinRichard M Schultz
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carl T BergstromRustom Antia

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