PMID: 13698150Dec 1, 1960Paper

The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group

The Journal of Experimental Medicine
F S FARAHH N Eisen

Abstract

Injection of rabbits with Freund's complete adjuvant containing bovine gamma-globulin substituted to near saturation with dinitrophenyl groups leads to the production of large amounts of antibodies specific for the 2,4-dinitrophenyl substituent. These antibodies can be isolated from sera in satisfactory yield (40 per cent) and in a high state of purity (90 per cent specifically precipitable). Hapten dissolution, which was used to screen haptens for their potential usefulness as specific reagents in the purification procedure, provides a means for evaluating, in relative terms, antibody-hapten affinities (in competition with antigen).

Citations

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