PMID: 698210Sep 11, 1978Paper

The preparation of large single bilayer liposomes by a fast and controlled dialysis

Biochimica Et Biophysica Acta
M H MilsmannH G Weder

Abstract

A new method is described for the preparation of large, homogeneously sized, single bilayer phospholipid vesicles. This method, based on a fast and controlled dialysis of sodium cholate from phosphatidylcholine/cholate mixed micelles, has the advantage of high yield of homogeneous vesicles avoiding any dilution and mechanical stress during preparation. Physicochemical properties of these vesicles are examined by several techniques and compared with those prepared with other methods.

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