The prereplication complex recruits XEco2 to chromatin to promote cohesin acetylation in Xenopus egg extracts

Current Biology : CB
Torahiko L HigashiTatsuro S Takahashi

Abstract

Sister chromatids are held together by the ring-shaped cohesin complex, which is loaded onto chromosomes before DNA replication. Cohesion between sister chromosomes is established during DNA replication, and it requires acetylation of the Smc3 subunit of cohesin by evolutionally conserved cohesin acetyltransferases (CoATs). However, how CoATs are recruited to chromatin and how cohesin acetylation is regulated remain unclear. We found that cohesin acetylation requires pre-RC-dependent chromatin loading of cohesin, but surprisingly, it is independent of DNA synthesis in Xenopus egg extracts. Immunodepletion experiments revealed that XEco2 is the CoAT responsible for Smc3 acetylation and sister chromatid cohesion. Recruitment of XEco2 onto chromatin was dependent on pre-RC assembly but was independent of cohesin loading and DNA synthesis. Two short N-terminal motifs, PBM-A and PBM-B, which are conserved among vertebrate Esco2/XEco2 homologs, were collectively essential for pre-RC-dependent chromatin association of XEco2, cohesin acetylation, and subsequent sister chromatid cohesion. The conserved PCNA-interacting protein box in XEco2 was largely dispensable for Smc3 acetylation but was partially required for cohesion. Interaction ...Continue Reading

References

Jul 14, 1998·Molecular Cell·J WalterJ Newport
Oct 21, 1998·Current Biology : CB·F Uhlmann, K Nasmyth
Mar 26, 2003·Cell·Stephan GruberKim Nasmyth
Sep 3, 2004·Current Biology : CB·Peter J Gillespie, Tatsuya Hirano
Sep 28, 2004·Nature Cell Biology·Tatsuro S TakahashiJohannes C Walter
Aug 29, 2006·Molecular Cell·George-Lucian MoldovanStefan Jentsch
Sep 12, 2006·Molecular Cell·Armelle LengronneFrank Uhlmann
Jul 17, 2008·Genes & Development·Tatsuro S TakahashiJohannes C Walter
Jul 26, 2008·Science·Tom Rolef Ben-ShaharFrank Uhlmann
Jul 26, 2008·Science·Elçin UnalDouglas E Koshland
Mar 10, 2009·Current Biology : CB·Takashi SutaniKatsuhiko Shirahige
Nov 6, 2009·Annual Review of Genetics·Kim Nasmyth, Christian H Haering
Jul 14, 2010·Trends in Cell Biology·Robert V Skibbens
Sep 14, 2010·Molecular Cell·Frederic BeckouëtKim Nasmyth
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrea L LafontSusannah Rankin
Nov 30, 2010·Cell·Tomoko NishiyamaJan-Michael Peters
Dec 17, 2010·Genes & Development·Emmanuelle GuillouJuan Méndez
May 10, 2011·Molecular Cell·Nicholas A Lyons, David O Morgan
Oct 5, 2011·Nature Cell Biology·Kim Nasmyth

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Citations

May 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Vladimir P BermudezJerard Hurwitz
Dec 25, 2013·PLoS Genetics·Robert V SkibbensEden E Tanzosh
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sadia RahmanPrasad V Jallepalli
Oct 22, 2013·The EMBO Journal·María CarreteroAna Losada
Jun 12, 2013·The Plant Journal : for Cell and Molecular Biology·Pablo Bolaños-VillegasGuang-Yuh Jauh
May 18, 2013·The FEBS Journal·Atsuhiro ShimadaKenji Fukui
Oct 15, 2014·Developmental Cell·Judith H I HaarhuisBenjamin D Rowland
Jul 25, 2015·Journal of Cellular Physiology·Grazia FazioValentina Massa
Nov 26, 2013·Biochimica Et Biophysica Acta·Alexander R BallKyoko Yokomori
Dec 12, 2012·Current Opinion in Cell Biology·Silvia Remeseiro, Ana Losada
Apr 15, 2016·Nature Reviews. Molecular Cell Biology·Frank Uhlmann
Sep 16, 2014·Current Biology : CB·Rene LadurnerJan-Michael Peters
Jul 13, 2016·ELife·Yoshitaka KawasoeTatsuro S Takahashi
Aug 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Reem M AlomerSusannah Rankin
Jan 10, 2019·Life Science Alliance·Akiyo HayashiHideo Nishitani
Aug 17, 2012·The Journal of Biological Chemistry·Jianhua SongSusannah Rankin
Mar 16, 2017·Scientific Reports·William C H ChaoMartin R Singleton
Dec 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Dawn BenderSusannah Rankin
Jun 20, 2020·Scientific Reports·Tahereh AjamGregor Eichele
Dec 14, 2018·Current Opinion in Cell Biology·Tomoko Nishiyama
Mar 24, 2021·Gene·Atsunori YoshimuraKatsuhiko Shirahige
Apr 4, 2021·International Journal of Molecular Sciences·Ana BoavidaFrancesca M Pisani

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