The presence of a high-Km hexokinase activity in dog, but not in boar, sperm

FEBS Letters
Josep M FernAndez-NovellJoan E Rodríguez-Gil

Abstract

The presence of a high-Km hexokinase activity was tested in both dog and boar spermatozoa. Hexokinase kinetics from dog extracts showed the presence of a specific activity (dog-sperm glucokinase-like protein, DSGLP), in the range of glucose concentrations of 4-10 mM, whereas boar sperm did not show any DSGLP activity. Furthermore, dog-sperm cells, but not those of boar, showed the presence of a protein which specifically reacted against a rat-liver anti-glucokinase antibody. This protein also had a molecular weight equal to that observed in rat-liver extracts, suggesting a close similarity between both the proteins. This glucokinase-like protein was distributed in the peri- and post-acrosomal zones of the head, and the midpiece and principal piece of tail of dog spermatozoa. These results indicate that dog spermatozoa have functional high-Km hexokinase activity, which could contribute to a very fine regulation of their hexose metabolism. This strict regulation could ultimately be very important in optimizing dog-sperm function along its life-time.

References

Sep 25, 1998·The Journal of Biological Chemistry·F RencurelA Leturque
May 17, 2000·Molecular Reproduction and Development·J BallesterJ E Rodríguez-Gil
Mar 27, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·T RigauJ E Rodríguez-Gil
Jan 28, 2003·Molecular Reproduction and Development·Maria J PalomoJoan E Rodríguez-Gil
Jul 19, 2003·Science·Joseph GrimsbyJoseph F Grippo
Nov 19, 2003·FEBS Letters·Silvia MarinMarta Cascante

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Citations

Mar 24, 2011·Reproduction, Fertility, and Development·J M Fernández-NovellJ E Rodríguez-Gil
May 3, 2014·BioMed Research International·Alessandra Ferramosca, Vincenzo Zara
Jul 23, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Yuki KatohNaomichi Okamura
Oct 10, 2007·Molecular Reproduction and Development·Noriko NakamuraEdward M Eddy
Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·J E Rodriguez-Gil
Dec 20, 2005·Molecular Reproduction and Development·Antonio MedranoJoan E Rodríguez-Gil
Dec 6, 2008·Reproduction in Domestic Animals = Zuchthygiene·D BucciG Galeati
Jun 22, 2015·Theriogenology·Joan E Rodríguez-Gil, Sergi Bonet

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